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  • 1
    In: Biodiversitas Journal of Biological Diversity, UNS Solo, Vol. 23, No. 10 ( 2022-11-02)
    Abstract: Abstract. Afililla Z, Suwanti LT, Mufasirin, Koerniawan HP, Suyono EA, Budiman A, Siregar UJ. 2022. The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro. Biodiversitas 23: 5205-5209. Indonesia is known as a maritime country that has various organisms, including microalgae. Some microalgae have many bioactivities, such as immunomodulatory activity. This study aimed to determine Glagah consortium microalgae polysaccharide (GCMP) and polysaccharide of Spirulina platensis (PSP) as an immunomodulator based on its effect on splenocyte proliferation and cytokine production in vitro. Crude polysaccharides were extracted by lye extraction method. Splenocytes were obtained from spleen of healthy mice and cultured in RPMI 1640 medium with and without Concanavalin A (Con A). Cells (3×105 per well) were treated with polysaccharide with dose gradually 0, 1.25, 2.5, 10 and 20 µg/mL. Splenocytes proliferation were observed by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. The supernatant was taken for the detection of cytokines using enzyme-linked immunoassay (ELISA). The results showed that GCMP, both with and without Con A at a concentration of less than 10 mg/mL decreased cell viability, on the other hand, a concentration of 10-20 mg/mL had a proliferative effect. Splenocytes were shown a proliferative effect at all concentrations of PSP, both with and without Con A. The concentration of polysaccharides, both GCMP and PSP, did not affect cytokine levels. In conclusion, based on the proliferation of splenocytes, GCMP has an immunomodulatory effect depending on its concentration.
    Type of Medium: Online Resource
    ISSN: 2085-4722 , 1412-033X
    Language: Unknown
    Publisher: UNS Solo
    Publication Date: 2022
    detail.hit.zdb_id: 2660049-3
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  • 2
    Online Resource
    Online Resource
    Universitas Airlangga ; 2021
    In:  Media Kedokteran Hewan Vol. 32, No. 3 ( 2021-09-07), p. 105-
    In: Media Kedokteran Hewan, Universitas Airlangga, Vol. 32, No. 3 ( 2021-09-07), p. 105-
    Abstract: Salah satu permasalahan yang sering dihadapi para peternak kerbau adalah penyakit Fascioliasis yang disebabkan oleh cacing hati atau Fasciola spp., dimana penyakit tersebut adalah salah satu penyakit yang bersifat zoonosis. Oleh sebab itu penelitian ini bertujuan untuk mengetahui prevalensi Fasciola spp. pada kerbau di Kabupaten Agam, Sumatra Barat. Sampel berupa feses 105 ekor kerbau dari tiga kecamatan lalu diperiksa dengan menggunakan metode natif (sederhana) dan sedimentasi sederhana. Hasil penelitian diperoleh 25 ekor dari 105 ekor kerbau dinyatakan positif terinfeksi Fasciola spp. Prevalensi yang diperoleh secara keseluruhan adalah 23,81% (25/105), dan hasil ini dapat dikembangkan dalam penelitian lebih lanjut.
    Type of Medium: Online Resource
    ISSN: 2775-975X , 0215-8930
    Language: Unknown
    Publisher: Universitas Airlangga
    Publication Date: 2021
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  • 3
    In: Journal of Parasite Science, Universitas Airlangga, Vol. 2, No. 1 ( 2019-12-02), p. 15-
    Abstract: Penelitian ini bertujuan untuk mengetahui prevalensi dan tingkat keparahan scabies (Sarcoptes scabiei) pada ternak kelinci di Desa Sajen, Kecamatan Pacet, Kabupaten Mojokereto. Sarcoptes scabiei var. cuniculi merupakan tungau dengan predileksi di stratum korneum dari kulit dan penyebab scabies. Gejala klinis ditandai dengan alopecia and krusta pada kulit. Penelitian ini menggunakan metode penelitian eksploratif labolatorik dengan pendekatan cross sectional. Sampel yang digunakan adalah kelinci pada satu peternakan yang memiliki gejala klinis scabies dan hasil dari kerokan kulit pada pemeriksaan laboratoris secara natif. Kelinci yang   positif scabies kemudian dilakukan skoring berdasarkan tingkat keparahan pada regio tubuh dan gejala klinisnya. Hasil penelitian menunjukkan bahwa dari 110 ekor kelinci dalam populasi, terdapat 19 ekor yang terinfeksi scabies dan terdapat 2 tingkat keparahan scabies pada kelinci yaitu tingkat keparahan ringan dan sedang yang terbagi atas 2 regio tubuh. Tingkat keparahan ringan menunjukkan gejala klinis alopecia dan krusta pada regio wajah sedangkan tingkat keparahan sedang pada regio wajah dan kaki. Kesimpulan pada penelitian ini adalah angka prevalensi scabies sebesar 17,27% dan tingkat keparahan ringan sebesar 26,32% serta tingkat keparahan sedang sebesar 73,68.
    Type of Medium: Online Resource
    ISSN: 2656-5331 , 2599-0993
    Language: Unknown
    Publisher: Universitas Airlangga
    Publication Date: 2019
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  • 4
    Online Resource
    Online Resource
    Universitas Airlangga ; 2017
    In:  Indonesian Journal of Tropical and Infectious Disease Vol. 6, No. 6 ( 2017-12-21), p. 154-
    In: Indonesian Journal of Tropical and Infectious Disease, Universitas Airlangga, Vol. 6, No. 6 ( 2017-12-21), p. 154-
    Abstract: Helminthiasis can be transmitted from animals to humans (zoonosis). Helminthiasis can cause cutaneus larva migrants, visceral larva migrant, and occular larva migrants. Cats are the most easily animals can found in public areas. cats have a habit of defecating in areas, such as dusty soil, gardens, sand pits, trash cans, and even children’s playgrounds. Proximity of human life with a stray cats is one of the potential that can helminthiasis transmited to humans. The purpose of this study was to assess the prevalence of helminth eggs (species and number) observed in cat feces contaminating public areas in Surabaya. Cross-sectional study have been observations cats existense and examination laboratory of 180 cat fecal samples were collected from canteens, markets, villages, schools, and parks across 5 areas in Surabaya. Helminth eggs present in fecal samples were identified using direct smear, sedimentation, and flotation methodes, and quantified as fecal egg count (eggs per gram of feces) with McMasster method. The test results positive for helminthiasis if found one or more types of helminth eggs in fecal samples. Helminth eggs were present in 68 (37.8%) of the 180 cat fecal samples contaminating public areas in Surabaya. Results of chi-squared analysis confirmed the prevalence of helminth eggs in cat fecal samples contaminating canteen, markets, villages, schools, and parks in Surabaya (p 〉 0.05). The species causing environmental contamination included Ancylostoma sp. eggs,  Toxocara cati eggs, and Toxascaris leonina eggs. The level of environmental contamination, as assessed using ANOVA, was 200 eggs per gram of feces.
    Type of Medium: Online Resource
    ISSN: 2356-0991 , 2085-1103
    Language: Unknown
    Publisher: Universitas Airlangga
    Publication Date: 2017
    detail.hit.zdb_id: 3072854-X
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  • 5
    In: Biodiversitas Journal of Biological Diversity, UNS Solo, Vol. 23, No. 6 ( 2022-06-03)
    Abstract: Abstract. Fatmawati M, Suwanti LT, Mufasirin, Hambarruk W, Sukesi LE, Rofiah, Novianto E, Wahyuningtyas BK. 2022. A comparative study among dairy goat breeds in Lumajang and Malang, East Java based on milk organoleptic and milk composition. Biodiversitas 23: 2899-2903. The Regencies of Lumajang and Malang have the potential for local dairy goats to support food security and food safety. In Lumajang and Malang there are a diversity of dairy goats that breed Senduro, Crossbreed Etawa, Sapera, and Menggolo. This study aimed to compare the milk quality of various breeds of dairy goats in Lumajang and Malang regencies based on organoleptic and composition tests. The total sample was 161 milk samples from Lumajang (115 dairy goats) and Malang (56 dairy goats). The organoleptic test of milk was carried out by testing the freshness, smell, taste, viscosity, and color of the milk. The milk composition test used lactoscan. The analysis consisted of the Chi-Square test for organoleptic and the Kruskal Wallis for milk composition test. The results show that the quality of goat's milk is of premium quality. The order of milk quality based on fat content is milk from Menggolo, Senduro, Etawa, and Sapera breeds. This study concluded that the breed of dairy goat influence the composition and quality of goat's milk. The milk quality of all dairy goat breeds from Lumajang and Malang was premium quality, but the Menggolo goat is the best, so it can be developed to increase local food productivity.
    Type of Medium: Online Resource
    ISSN: 2085-4722 , 1412-033X
    Language: Unknown
    Publisher: UNS Solo
    Publication Date: 2022
    detail.hit.zdb_id: 2660049-3
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  • 6
    In: Parasitology International, Elsevier BV, Vol. 86 ( 2022-02), p. 102478-
    Type of Medium: Online Resource
    ISSN: 1383-5769
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2022
    detail.hit.zdb_id: 1498553-6
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  • 7
    Online Resource
    Online Resource
    Universitas Airlangga ; 2017
    In:  Indonesian Journal of Tropical and Infectious Disease Vol. 6, No. 6 ( 2017-12-21), p. 150-
    In: Indonesian Journal of Tropical and Infectious Disease, Universitas Airlangga, Vol. 6, No. 6 ( 2017-12-21), p. 150-
    Abstract: Scabies is a zoonotic skin disease caused by Sarcoptes scabiei mites. As an emerging/re-emerging parasitic disease, scabies represents a significant global threat to both human and animal health. Numerous cases of scabies in Indonesia have been reported, which support research on the prevalence of S. scabiei. However, most such studies have involved conventional morphological studies, with limited molecular diagnostic studies. The purpose of the present study was the genetic characterization of S. scabiei var. cuniculi in domestic rabbits to generate baseline genotypic data. S. scabiei var. cuniculi was isolated and identified from scabies-infected rabbits from the Surabaya and Malang regions of East Java. Molecular identification was performed using Polymerase Chain Reaction (PCR) using specific primers targeting the COX1 gene. We performed COX1 PCR using rabbit isolates of S. scabiei from Indonesia. To the best of our knowledge, no such study had been reported previously. This study was performed in the Laboratory of Veterinary Parasitology, Faculty of Veterinary Medicine and the Tropical Disease Diagnostic Center Laboratory, Universitas Airlangga. The results with agarose gel electrophoresis revealed a 289 bp PCR product amplified from the DNA of S. scabiei isolates from both Surabaya and Malang in accordance with the expected COX1 amplicon size, that indicated a single band 289 bp in length, demonstrating specific detection of S. scabiei var. cuniculi from Surabaya and Malang using COX1 primers. The results were consistent with the calculated amplicon size based on primer positions within the COX1 locus, with the forward primer spanning nucleotides 61–94, and the reverse primer spanning nucleotides 331–350 ( 350 − 61 = 289 bp).  PCR genotyping of the isolates yielded an identical nucleotide length of 289 bp. Further studies are required to sequence the amplified fragments for homology assessment.
    Type of Medium: Online Resource
    ISSN: 2356-0991 , 2085-1103
    Language: Unknown
    Publisher: Universitas Airlangga
    Publication Date: 2017
    detail.hit.zdb_id: 3072854-X
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  • 8
    In: Journal of Parasite Science, Universitas Airlangga, Vol. 2, No. 2 ( 2019-12-03), p. 61-
    Abstract: Research of gastrointestinal protozoan in cats at Surabaya are still limited. Protozoa infection of the gastrointestinal tract can cause diarrhea and even zoonosis. This research aimed to identify and determine the prevalence of protozoan in cats in animal hostptal and animal clinic in Surabaya. Ninety  fecal samples were collected from 2 animal clinic and one animal hospital. Samples were  examined e wet mount (native, sedimentation, and floatation) and (Ziehl Nellsen) stain. Protozoa was identified by using a light microscope with 400x and 1000x magnification. The result showed 68,89% of samples were positively infected by gastrointestinal protozoa. The protozoa were Blastocystis sp, Cryptosporidium sp, Giardia sp, and Eimeria sp. By chi square test, there was  not significant differences the prevalence of gastrointestinal protozoan in cat between sex, age, breed, and diarrhea status
    Type of Medium: Online Resource
    ISSN: 2656-5331 , 2599-0993
    Language: Unknown
    Publisher: Universitas Airlangga
    Publication Date: 2019
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  • 9
    In: Journal of Parasite Science, Universitas Airlangga, Vol. 3, No. 2 ( 2019-09-12), p. 83-
    Abstract: The purpose of this study is to determine the prevalence, infection level, sex and age effect on the infection level of GIT Nematode parasite in a horse. The fecal samples analyzed using the methods of native, sedimentation, floatation, and worm eggs count per gram of feces. Sample examination found 54 positive infected gastrointestinal nematode parasite with prevalence rate of 87% (54 from 62). The prevalence of Trichonema sp., Strongylus sp., and Parascaris equorum are 37.1%, 16.1%, and 1.6%. There were also mixed infestation like Strongylus sp. and Trichonema sp; Strongylus sp. and Parascaris equorum; Trichonema sp. and Parascaris equorum with total prevalence 27.4%, 1.6%, and 3.2%. Sex and age of horse had a very significant and significant effect in prevalence and infection level of Nematode parasite.
    Type of Medium: Online Resource
    ISSN: 2656-5331 , 2599-0993
    Language: Unknown
    Publisher: Universitas Airlangga
    Publication Date: 2019
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  • 10
    Online Resource
    Online Resource
    Universitas Airlangga ; 2019
    In:  Journal of Parasite Science Vol. 1, No. 2 ( 2019-11-26), p. 39-
    In: Journal of Parasite Science, Universitas Airlangga, Vol. 1, No. 2 ( 2019-11-26), p. 39-
    Abstract: The aim of this research was to investigate the number of prevalence of Trypanosomiasis of wild rats in Banyuwangi. Sixty wild rats were trapped from human residence, markets and rice fields in Banyuwangi. Rat`s  blood smear was stained by Giemsa. The result show that one (1.67%.) of 60 blood sample was appear Trypanosoma sp..  The prevalence of Trypanosomiasis of wild rats in Banyuwangi was 1.67%.
    Type of Medium: Online Resource
    ISSN: 2656-5331 , 2599-0993
    Language: Unknown
    Publisher: Universitas Airlangga
    Publication Date: 2019
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