In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 17, No. 4 ( 2021-4-28), p. e1009507-
Abstract:
The development of a universal vaccine against foot-and-mouth disease virus (FMDV) is hindered by cross-serotype antigenic diversity and by a lack of knowledge regarding neutralization of the virus in natural hosts. In this study, we isolated serotype O-specific neutralizing antibodies (NAbs) (F145 and B77) from recovered natural bovine hosts by using the single B cell antibody isolation technique. We also identified a serotype O/A cross-reacting NAb (R50) and determined virus-NAb complex structures by cryo-electron microscopy at near-atomic resolution. F145 and B77 were shown to engage the capsid of FMDV-O near the icosahedral threefold axis, binding to the BC/HI-loop of VP2. In contrast, R50 engages the capsids of both FMDV-O and FMDV-A between the 2- and 5-fold axes and binds to the BC/EF/GH-loop of VP1 and to the GH-loop of VP3 from two adjacent protomers, revealing a previously unknown antigenic site. The cross-serotype neutralizing epitope recognized by R50 is highly conserved among serotype O/A. These findings help to elucidate FMDV neutralization by natural hosts and provide epitope information for the development of a universal vaccine for cross-serotype protection against FMDV.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1009507
DOI:
10.1371/journal.ppat.1009507.g001
DOI:
10.1371/journal.ppat.1009507.g002
DOI:
10.1371/journal.ppat.1009507.g003
DOI:
10.1371/journal.ppat.1009507.g004
DOI:
10.1371/journal.ppat.1009507.g005
DOI:
10.1371/journal.ppat.1009507.g006
DOI:
10.1371/journal.ppat.1009507.s001
DOI:
10.1371/journal.ppat.1009507.s002
DOI:
10.1371/journal.ppat.1009507.s003
DOI:
10.1371/journal.ppat.1009507.s004
DOI:
10.1371/journal.ppat.1009507.s005
DOI:
10.1371/journal.ppat.1009507.s006
DOI:
10.1371/journal.ppat.1009507.s007
DOI:
10.1371/journal.ppat.1009507.s008
DOI:
10.1371/journal.ppat.1009507.s009
DOI:
10.1371/journal.ppat.1009507.s010
DOI:
10.1371/journal.ppat.1009507.s011
DOI:
10.1371/journal.ppat.1009507.s012
DOI:
10.1371/journal.ppat.1009507.s013
DOI:
10.1371/journal.ppat.1009507.s014
DOI:
10.1371/journal.ppat.1009507.s015
DOI:
10.1371/journal.ppat.1009507.s016
DOI:
10.1371/journal.ppat.1009507.s017
DOI:
10.1371/journal.ppat.1009507.s018
DOI:
10.1371/journal.ppat.1009507.s019
DOI:
10.1371/journal.ppat.1009507.s020
DOI:
10.1371/journal.ppat.1009507.r001
DOI:
10.1371/journal.ppat.1009507.r002
DOI:
10.1371/journal.ppat.1009507.r003
DOI:
10.1371/journal.ppat.1009507.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2205412-1
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