In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 70, No. 8_Supplement ( 2010-04-15), p. 1356-1356
Abstract:
We imaged the interaction between osteosarcoma cells and splenocytes using fluorescent proteins during chemotherapy or dendritic-cell (DC) therapy. GFP-expressing splenocytes (5 × 106), derived from GFP transgenic BALB/c mice, were transplanted to BALB/c nude mice. Then, RFP-143B human osteosarcoma cells (5 × 105) were injected in a skin flap raised in these mice. The mice were treated with doxorubicin (5 mg/kg), i.p. three days in a row. The RFP-expressing 143B tumor and the GFP splenocytes accumulation around the tumor were imaged for three days. Dendritic cells (1×106), which were activated with a 143B cells lysate, were injected into the inguinal lymph node of the mice. The tumor grew gradually in the control group, whereas in the chemotherapy group, the tumor became progressively smaller. In the DC group, the tumor regressed and was almost eradicated 12 days after treatment. In the DC group, GFP splenocytes accumulated in large numbers around the tumor three days after DC treatment. The accumulation of DC cells was diminished as the tumor became smaller. In the chemotherapy group, in contrast, accumulation of splenocytes gradually increased even though the tumor became progressively smaller. Future experiments will be on syngeneic models to determine the influence of an intact immune system on the interaction of splenocytes and DC cells with cancer cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1356.
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM10-1356
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2010
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
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