In:
Angewandte Chemie, Wiley, Vol. 134, No. 35 ( 2022-08-26)
Abstract:
The complexation of Mg II with adenosine 5′‐triphosphate (ATP) is omnipresent in biochemical energy conversion, but is difficult to interrogate directly. Here we use the spin‐ β‐emitter 31 Mg to study Mg II ‐ATP complexation in 1‐ethyl‐3‐methylimidazolium acetate (EMIM‐Ac) solutions using β‐radiation‐detected nuclear magnetic resonance (β‐NMR). We demonstrate that (nuclear) spin‐polarized 31 Mg, following ion‐implantation from an accelerator beamline into EMIM‐Ac, binds to ATP within its radioactive lifetime before depolarizing. The evolution of the spectra with solute concentration indicates that the implanted 31 Mg initially bind to the solvent acetate anions, whereafter they undergo dynamic exchange and form either a mono‐ ( 31 Mg‐ATP) or di‐nuclear ( 31 MgMg‐ATP) complex. The chemical shift of 31 Mg‐ATP is observed up‐field of 31 MgMg‐ATP, in accord with quantum chemical calculations. These observations constitute a crucial advance towards using β‐NMR to probe chemistry and biochemistry in solution.
Type of Medium:
Online Resource
ISSN:
0044-8249
,
1521-3757
DOI:
10.1002/ange.v134.35
DOI:
10.1002/ange.202207137
Language:
English
Publisher:
Wiley
Publication Date:
2022
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