In:
Frontiers in Cellular and Infection Microbiology, Frontiers Media SA, Vol. 13 ( 2023-6-19)
Abstract:
Many Gram-negative bacteria use quorum sensing (QS) signal molecules to monitor their local population density and to coordinate their collective behaviors. The diffusible signal factor (DSF) family represents an intriguing type of QS signal to mediate intraspecies and interspecies communication. Recently, accumulating evidence demonstrates the role of DSF in mediating inter-kingdom communication between DSF-producing bacteria and plants. However, the regulatory mechanism of DSF during the Xanthomonas -plant interactions remain unclear. Methods Plants were pretreated with different concentration of DSF and subsequent inoculated with pathogen Xanthomonas campestris pv. campestris (Xcc) . Pathogenicity, phynotypic analysis, transcriptome combined with metabolome analysis, genetic analysis and gene expression analysis were used to evaluate the priming effects of DSF on plant disease resistance. Results We found that the low concentration of DSF could prime plant immunity against Xcc in both Brassica oleracea and Arabidopsis thaliana . Pretreatment with DSF and subsequent pathogen invasion triggered an augmented burst of ROS by DCFH-DA and DAB staining. CAT application could attenuate the level of ROS induced by DSF. The expression of RBOHD and RBOHF were up-regulated and the activities of antioxidases POD increased after DSF treatment followed by Xcc inoculation. Transcriptome combined with metabolome analysis showed that plant hormone jasmonic acid (JA) signaling involved in DSF-primed resistance to Xcc in Arabidopsis. The expression of JA synthesis genes ( AOC2, AOS, LOX2, OPR3 and JAR1 ), transportor gene ( JAT1 ), regulator genes ( JAZ1 and MYC2 ) and responsive genes ( VSP2, PDF1.2 and Thi2.1 ) were up-regulated significantly by DSF upon Xcc challenge. The primed effects were not observed in JA relevant mutant coi1-1 and jar1-1 . Conclusion These results indicated that DSF-primed resistance against Xcc was dependent on the JA pathway. Our findings advanced the understanding of QS signal-mediated communication and provide a new strategy for the control of black rot in Brassica oleracea .
Type of Medium:
Online Resource
ISSN:
2235-2988
DOI:
10.3389/fcimb.2023.1203582
DOI:
10.3389/fcimb.2023.1203582.s001
DOI:
10.3389/fcimb.2023.1203582.s002
DOI:
10.3389/fcimb.2023.1203582.s003
DOI:
10.3389/fcimb.2023.1203582.s004
DOI:
10.3389/fcimb.2023.1203582.s005
DOI:
10.3389/fcimb.2023.1203582.s006
DOI:
10.3389/fcimb.2023.1203582.s007
DOI:
10.3389/fcimb.2023.1203582.s008
DOI:
10.3389/fcimb.2023.1203582.s009
DOI:
10.3389/fcimb.2023.1203582.s010
DOI:
10.3389/fcimb.2023.1203582.s011
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2023
detail.hit.zdb_id:
2619676-1
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