In:
Frontiers in Genetics, Frontiers Media SA, Vol. 12 ( 2021-11-15)
Abstract:
Melatonin acts both as an antioxidant and as a growth regulatory substance in plants. Pseudomonas fluorescens endophytic bacterium has been shown to produce melatonin and increase plant resistance to abiotic stressors through increasing endogenous melatonin. However, in bacteria, genes are still not known to be melatonin-related. Here, we reported that the bacterial phenylalanine 4-hydroxylase (PAH) may be involved in the 5-hydroxytryptophan (5-HTP) biosynthesis and further influenced the subsequent production of melatonin in P. fluorescens . The purified PAH protein of P. fluorescens not only hydroxylated phenylalanine but also exhibited l -tryptophan ( l -Trp) hydroxylase activity by converting l -Trp to 5-HTP in vitro . However, bacterial PAH displayed lower activity and affinity for l -Trp than l -phenylalanine. Notably, the PAH deletion of P. fluorescens blocked melatonin production by causing a significant decline in 5-HTP levels and thus decreased the resistance to abiotic stress. Overall, this study revealed a possible role for bacterial PAH in controlling 5-HTP and melatonin biosynthesis in bacteria, and expanded the current knowledge of melatonin production in microorganisms.
Type of Medium:
Online Resource
ISSN:
1664-8021
DOI:
10.3389/fgene.2021.746392
DOI:
10.3389/fgene.2021.746392.s001
DOI:
10.3389/fgene.2021.746392.s002
DOI:
10.3389/fgene.2021.746392.s003
DOI:
10.3389/fgene.2021.746392.s004
DOI:
10.3389/fgene.2021.746392.s005
DOI:
10.3389/fgene.2021.746392.s006
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2606823-0
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