In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 71, No. 8_Supplement ( 2011-04-15), p. 1411-1411
Abstract:
Gastric cancer is the most common types of human cancer with an annual incidence of approximately 870,000 new cases worldwide (Crew K D, Neugut A I, Epidemiology of gastric cancer, World J Gastroenterol 2006;12(3):354-362). Despite its high prevalence, the risk factors and tumorigenesis of gastric cancer are not clearly identified. Consequently, the identification of the oncogene or tumour suppressor gene in gastric cancer has been the focus of many researches. In our effort to find gastric cancer related genes, we analysed the gene expression profiles in 30 pairs of gastric cancer patient samples (tumour and normal tissue) using microarrays and identified interferon-induced transmembrane protein 1 (IFITM1) as a highly up-regulated gene in tumor tissues. IFITM1, also known as 9-27 or Leu13, is a cell surface molecule and the IFN-inducible transmembrane protein family. However, there have been a few papers of IFITM1 explaining its function, and the role of IFITM1 in cancer is poorly understood. In the present study, we examined the roles of IFITM1 for gastric cancer and its function for tumoringenesis. Quantitative real-time PCR, western blot analysis and immunohistochemical staining were performed to confirm the cancer specific expression pattern of IFITM1. In various gastric cancer cell lines, IFITM1 mRNA expression level was highly up-regulated in comparison human mammary epithelial cell (HMEC). Furthermore, mRNA expression of IFITM1 was significantly up-regulated in cancer tissues than matching normal tissues. In addition, IFITM1 overexpression was observed in gastric adenocarcinoma by immunohistochemistry. Interestingly, cells which overexpressed IFITM1 exhibit an increased migration, invasion and wound healing potential. Consistently, IFITM1 knockdown cells by siRNA showed a decreased invasiveness. Our findings suggest that IFITM1 plays an important role for the migration, invasion and wound healing capacity of cancer cells and associated with progression of gastric cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1411. doi:10.1158/1538-7445.AM2011-1411
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM2011-1411
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2011
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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