In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 69, No. 4 ( 2019-04-01), p. 1041-1046
Kurzfassung:
A Gram-stain-negative, motile bacterium, designated strain YE3 T , was isolated from activated sludge obtained from a municipal wastewater treatment plant in Daejeon Metropolitan City, Republic of Korea. The cells were oxidase- and catalase-positive, and grew under aerobic conditions at 10–40 °C (optimum, 30 °C), with 1.0–8.0 % (w/v) NaCl (1.0 %) and at pH 5.5–9.0 (pH 7.0). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YE3 T was most closely related to Pusillimonas harenae KACC 14927 T (98.2 % sequence similarity) and Pusillimonas ginsengisoli KCTC 22046 T (98.0 %). DNA–DNA relatedness values for strain YE3 T and P. harenae KACC 14927 T , P. ginsengisoli KCTC 22046 T and P. soli KCTC 22455 T were 28.7±2.27 %, 21.3±1.16 %, and 14.0±0.67 %, respectively. The genomic G+C content of the type strain YE3 T was 59.3 mol%, as determined by whole-genome sequencing. The dominant fatty acids were C 16 : 0 (39.2 %) and C 17 : 0 cyclo (37.5 %). The major polar lipids of strain YE3 T were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Two aminophospholipids and four unidentified lipids were also detected. Furthermore, strain YE3 T was able to oxidize thiosulfate under heterotrophic conditions. Based on the phenotypic, genotypic, chemotaxonomic and phylogenetic analyses, strain YE3 T represents a novel species of the genus Pusillimonas , for which the name Pusillimonas thiosulfatoxidans sp. nov. is proposed. The type strain is YE3 T (=KCTC 62737 T =NBRC 113113 T ).
Materialart:
Online-Ressource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.003266
Sprache:
Englisch
Verlag:
Microbiology Society
Publikationsdatum:
2019
ZDB Id:
215062-1
ZDB Id:
2056611-6
SSG:
12
Permalink