In:
Journal of Oral Pathology & Medicine, Wiley, Vol. 48, No. 2 ( 2019-02), p. 136-142
Abstract:
To investigate alterations in histone modification and histone deacetylases ( HDAC s) in patients with oral lichen planus ( OLP ), and to evaluate correlations with inflammatory cytokine production. Methods Global histone H3/H4 acetylation and HDAC activity in CD 4+ T cells from 23 patients with OLP and 10 healthy control subjects were examined using spectrophotometry. The mRNA levels of eight members of four classes of HDAC genes were measured by real‐time quantitative polymerase chain reaction. Forty cytokines involved in inflammation were examined with a cytokine array. The correlation between histone modification and cytokine production was analyzed. Results Global histone H3 hypo‐acetylation was observed in OLP patients. Patients with OLP had significantly higher HDAC s activity,and higher HDAC 6 and HDAC 7 m RNA level compared with the controls. Of the 40 cytokines in the cytokine array, eight were significantly increased in OLP patients: interleukin ( IL )‐4, IL ‐8, IL ‐1ra, tumor necrosis factor receptor II ( TNFR II ), macrophage inflammatory protein 1b ( MIP ‐1b), fibrosis‐associated tissue inhibitors of metalloproteinase 1 ( TIMP )‐1, monocyte chemotactic protein 1 ( MCP ‐1), and eotaxin‐2. In the OLP group, the acetylation level of histone H3 was negatively correlated with IL ‐4 and MCP ‐1 production, and the expression of HDAC 6 mRNA was positively correlated with MCP ‐1 production. In the non‐erosive subgroup, acetylation of histone H3 was negatively correlated with IL ‐4, IL ‐16, and TIMP ‐2 production. In the erosive OLP subgroup, the expression of HDAC 7 mRNA was positively correlated with MIP ‐1a production. Conclusion Aberrant histone modification of CD 4+ T cells in peripheral blood could occur in OLP patients, and possibly affects inflammatory cytokine production.
Type of Medium:
Online Resource
ISSN:
0904-2512
,
1600-0714
DOI:
10.1111/jop.2019.48.issue-2
Language:
English
Publisher:
Wiley
Publication Date:
2019
detail.hit.zdb_id:
2026385-5
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