In:
Journal of Leukocyte Biology, Oxford University Press (OUP), Vol. 70, No. 2 ( 2001-08-01), p. 306-312
Abstract:
Endothelin (ET)-1(1-31) is a novel 31-amino acid-length peptide derived from big ET-1 by chymase or other chymotrypsin-type proteases and is a major ET derivative in human neutrophils. In this study, we revealed that ET-1(1-31), but not big ET, exhibited chemotactic activities toward human neutrophils and monocytes as an inflammatory mediator, although the effects were less potent than those of formyl-methionyl-leucyl-phenylalanine or interleukin-8. However, the chemotactic effects of ET-1(1-31) were much greater than those of the 21-amino acid ET-1, ET-1(1-21). Checkerboard analyses revealed that the effects are chemotactic rather than chemokinetic. The effects of ET-1(1-31) are not mediated by interleukin-8 or monocyte chemoattractant protein-1. The chemotactic effects and an increase in intracellular-free Ca2+ caused by ET-1(1-31) were significantly inhibited by BQ123, an ETA receptor antagonist, but not by BQ788, an ETB receptor antagonist, suggesting that ET-1(1-31) mediates chemotaxis through an ETA or ETA-like receptor.
Type of Medium:
Online Resource
ISSN:
0741-5400
,
1938-3673
DOI:
10.1189/jlb.70.2.306
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2001
detail.hit.zdb_id:
2026833-6
SSG:
12
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