In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 15, No. 1 ( 2021-1-25), p. e0008996-
Abstract:
Bovine tuberculosis (TB) caused by Mycobacterium bovis is a significant health threat to cattle and a zoonotic threat for humans in many developing countries. Rapid and accurate detection of M . bovis is fundamental for controlling the disease in animals and humans, and for the proper treatment of patients as one of the first-line anti-TB drug, pyrazinamide, is ineffective against M . bovis . Currently, there are no rapid, simplified and low-cost diagnostic methods that can be easily integrated for use in many developing countries. Here, we report the development of a loop-mediated isothermal amplification (LAMP) assay for specific identification of M . bovis by targeting the region of difference 4 (RD4), a 12.7 kb genomic region that is deleted solely in M . bovis . The assay's specificity was evaluated using 139 isolates comprising 65 M . bovis isolates, 40 M . tuberculosis isolates, seven M . tuberculosis complex reference strains, 22 non-tuberculous mycobacteria and five other bacteria. The established LAMP detected only M . bovis isolates as positive and no false positives were observed using the other mycobacteria and non-mycobacteria tested. Our LAMP assay detected as low as 10 copies of M . bovis genomic DNA within 40 minutes. The procedure of LAMP is simple with an incubation at a constant temperature. Results are observed with the naked eye by a color change, and there is no need for expensive equipment. The established LAMP can be used for the detection of M . bovis infections in cattle and humans in resource-limited areas.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0008996
DOI:
10.1371/journal.pntd.0008996.g001
DOI:
10.1371/journal.pntd.0008996.g002
DOI:
10.1371/journal.pntd.0008996.g003
DOI:
10.1371/journal.pntd.0008996.t001
DOI:
10.1371/journal.pntd.0008996.t002
DOI:
10.1371/journal.pntd.0008996.t003
DOI:
10.1371/journal.pntd.0008996.s001
DOI:
10.1371/journal.pntd.0008996.s002
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2429704-5
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