In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 4 ( 2021-4-27), p. e0250109-
Abstract:
Idiopathic pulmonary fibrosis (IPF) and lung cancer share common risk factors, epigenetic and genetic alterations, the activation of similar signaling pathways and poor survival. The aim of this study was to examine the gene expression profiles of stromal cells from patients with IPF and lung adenocarcinoma (ADC) as well as from normal lung. The gene expression levels of cultured stromal cells derived from non-smoking patients with ADC from the tumor (n = 4) and the corresponding normal lung (n = 4) as well as from patients with IPF (n = 4) were investigated with Affymetrix microarrays. The expression of collagen type IV alpha 1 chain, periostin as well as matrix metalloproteinase-1 and -3 in stromal cells and lung tissues were examined with quantitative real-time reverse transcriptase polymerase chain reaction and immunohistochemistry, respectively. Twenty genes were similarly up- or down-regulated in IPF and ADC compared to control, while most of the altered genes in IPF and ADC were differently expressed, including several extracellular matrix genes. Collagen type IV alpha 1 chain as well as matrix metalloproteinases-1 and -3 were differentially expressed in IPF compared to ADC. Periostin was up-regulated in both IPF and ADC in comparison to control. All studied factors were localized by immunohistochemistry in stromal cells within fibroblast foci in IPF and stroma of ADC. Despite the similarities found in gene expressions of IPF and ADC, several differences were also detected, suggesting that the molecular changes occurring in these two lung illnesses are somewhat different.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0250109
DOI:
10.1371/journal.pone.0250109.g001
DOI:
10.1371/journal.pone.0250109.g002
DOI:
10.1371/journal.pone.0250109.t001
DOI:
10.1371/journal.pone.0250109.t002
DOI:
10.1371/journal.pone.0250109.t003
DOI:
10.1371/journal.pone.0250109.t004
DOI:
10.1371/journal.pone.0250109.t005
DOI:
10.1371/journal.pone.0250109.s001
DOI:
10.1371/journal.pone.0250109.s002
DOI:
10.1371/journal.pone.0250109.s003
DOI:
10.1371/journal.pone.0250109.s004
DOI:
10.1371/journal.pone.0250109.s005
DOI:
10.1371/journal.pone.0250109.s006
DOI:
10.1371/journal.pone.0250109.s007
DOI:
10.1371/journal.pone.0250109.s008
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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