In:
Plant Disease, Scientific Societies
Abstract:
Gray mold, caused by Botrytis spp., is a serious problem in Norway spruce seedling production in forest nurseries. From 2013 to 2019, 125 isolates of Botrytis were obtained from eight forest nurseries in Norway: 53 from Norway spruce seedlings, 16 from indoor air, 52 from indoor surfaces, and four from weeds growing close to seedlings. A total of 109 isolates were identified as B. cinerea, and 75 of these were characterized as Botrytis group S. Botrytis pseudocinerea isolates were obtained along with isolates producing DNA sequences that were similar to those of B. prunorum. Fungicide resistance was assessed with a mycelial growth assay and resistance was found for the following: boscalid (8.8%), fenhexamid (33.6%), fludioxonil (17.6%), pyraclostrobin (36.0%), pyrimethanil (13.6%), and thiophanate-methyl (50.4%). Many isolates (38.4 %) were resistant to two to six different fungicides. A selection of isolates was analyzed for the presence of known resistance-conferring mutations in the cytb, erg27, mrr1, sdhB, and tubA genes, and mutations leading to G143A, F412S, ΔL497, H272R, and E198A/F200Y were detected, respectively. Detection of fungicide resistance in Botrytis from Norway spruce and forest nursery facilities reinforces the necessity of employing resistance management strategies to improve control and delay development of fungicide resistance in the gray mold pathogens.
Type of Medium:
Online Resource
ISSN:
0191-2917
,
1943-7692
DOI:
10.1094/PDIS-01-23-0037-RE
Language:
English
Publisher:
Scientific Societies
Publication Date:
2023
detail.hit.zdb_id:
2042679-3
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