In:
PLOS Biology, Public Library of Science (PLoS), Vol. 21, No. 2 ( 2023-2-28), p. e3001926-
Abstract:
Radial glial cells (RGCs) are essential for the generation and organization of neurons in the cerebral cortex. RGCs have an elongated bipolar morphology with basal and apical endfeet that reside in distinct niches. Yet, how this subcellular compartmentalization of RGCs controls cortical development is largely unknown. Here, we employ in vivo proximity labeling, in the mouse, using unfused BirA to generate the first subcellular proteome of RGCs and uncover new principles governing local control of cortical development. We discover a cohort of proteins that are significantly enriched in RGC basal endfeet, with MYH9 and MYH10 among the most abundant. Myh9 and Myh10 transcripts also localize to endfeet with distinct temporal dynamics. Although they each encode isoforms of non-muscle myosin II heavy chain, Myh9 and Myh10 have drastically different requirements for RGC integrity. Myh9 loss from RGCs decreases branching complexity and causes endfoot protrusion through the basement membrane. In contrast, Myh10 controls endfoot adhesion, as mutants have unattached apical and basal endfeet. Finally, we show that Myh9- and Myh10 -mediated regulation of RGC complexity and endfoot position non-cell autonomously controls interneuron number and organization in the marginal zone. Our study demonstrates the utility of in vivo proximity labeling for dissecting local control of complex systems and reveals new mechanisms for dictating RGC integrity and cortical architecture.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001926
DOI:
10.1371/journal.pbio.3001926.g001
DOI:
10.1371/journal.pbio.3001926.g002
DOI:
10.1371/journal.pbio.3001926.g003
DOI:
10.1371/journal.pbio.3001926.g004
DOI:
10.1371/journal.pbio.3001926.g005
DOI:
10.1371/journal.pbio.3001926.g006
DOI:
10.1371/journal.pbio.3001926.g007
DOI:
10.1371/journal.pbio.3001926.g008
DOI:
10.1371/journal.pbio.3001926.t001
DOI:
10.1371/journal.pbio.3001926.s001
DOI:
10.1371/journal.pbio.3001926.s002
DOI:
10.1371/journal.pbio.3001926.s003
DOI:
10.1371/journal.pbio.3001926.s004
DOI:
10.1371/journal.pbio.3001926.s005
DOI:
10.1371/journal.pbio.3001926.s006
DOI:
10.1371/journal.pbio.3001926.s007
DOI:
10.1371/journal.pbio.3001926.s008
DOI:
10.1371/journal.pbio.3001926.s009
DOI:
10.1371/journal.pbio.3001926.s010
DOI:
10.1371/journal.pbio.3001926.s011
DOI:
10.1371/journal.pbio.3001926.s012
DOI:
10.1371/journal.pbio.3001926.s013
DOI:
10.1371/journal.pbio.3001926.s014
DOI:
10.1371/journal.pbio.3001926.s015
DOI:
10.1371/journal.pbio.3001926.s016
DOI:
10.1371/journal.pbio.3001926.s017
DOI:
10.1371/journal.pbio.3001926.s018
DOI:
10.1371/journal.pbio.3001926.s019
DOI:
10.1371/journal.pbio.3001926.s020
DOI:
10.1371/journal.pbio.3001926.r001
DOI:
10.1371/journal.pbio.3001926.r002
DOI:
10.1371/journal.pbio.3001926.r003
DOI:
10.1371/journal.pbio.3001926.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2126773-X
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