In:
Analytical Cellular Pathology, Hindawi Limited, Vol. 29, No. 1 ( 2007-01-01), p. 37-45
Abstract:
Background : Cell cycle proteins and HIF-1 α with downstream factors are often abberrantly expressed in (pre)neoplastic tissue. Methods : Paraffin-embedded specimens of inactive endometrium with TM ( n =15), ovarian inclusion cysts ( n =6), cervix with TM (tubal metaplasia) ( n =3), Fallopian tubes ( n =7), cycling endometrium ( n =9) and a ciliated cell tumor of the ovary were stained for p16 and LhS28. 39 Endometrioid endometrial carcinomas and 5 serous endometrial carcinomas were stained for p16. Additionally, inactive endometrium ( n =15) was immunohistochemically stained for p21, p27, p53, cyclin A, cyclin D1, cyclin E, HIF-1 α , CAIX, Glut-1 and MIB-1. Results : A mosaic pattern of expression of p16 was seen throughout in all cases of endometrial TM (15/15), in 2/6 of the ovarian inclusion cysts with TM, in all (3/3) cervical TM and focal in 5/7 of Fallopian tube cases. Mosaic expression was also seen in a ciliated cell tumor of the ovary and in 18/39 of endometrioid endometrial carcinomas, and diffuse p16 expression was seen in 5/5 serous carcinomas. In comparison with normal endometrium, TM areas in the endometrium showed significantly increased expression of HIF-1 α , cyclin E, p21 and cyclin A, and decreased expression of p27. Membranous expression of CAIX and Glut-1 was only seen in TM areas, pointing to functional HIF-1 α . Conclusion : As p16 is consistently expressed in TM, less and only patchy expressed in the normal Fallopian tube, is paralleled by aberrant expression of cell cycle proteins, HIF-1 α , CAIX and Glut-1 and resembles the pattern of p16 expression frequently seen in endometrial carcinomas, we propose endometrial TM to be a potential premalignant endometrial lesion.
Type of Medium:
Online Resource
ISSN:
2210-7177
,
2210-7185
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2007
detail.hit.zdb_id:
2584078-2
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