In:
The EMBO Journal, EMBO, Vol. 36, No. 13 ( 2017-07-03), p. 1888-1907
Abstract:
image Stimulation of cAMP signaling provides a means to in vitro culturing competent primordial germ cell‐like cells ( PGCLC s), which has remained a major challenge in reproductive biology, and allows the characterization of their epigenetic and transcriptional states, mirroring those occurring during gonadal germ cell development. cAMP signaling stimulation by forskolin and rolipram increases in vitro proliferation of mouse embryonic stem cell‐derived PGCLCs up to ˜50 fold. Expanded PGCLCs continue to proliferate in culture beyond day 6 and contribute robustly to spermatogenesis and fertile offspring in vivo after transplantation. Expanded PGCLCs maintain their transcriptome and histone modification states, analogous to a sexually uncommitted PGC state. Expanded PGCLCs comprehensively erase their DNA methylome.
Type of Medium:
Online Resource
ISSN:
0261-4189
,
1460-2075
DOI:
10.15252/embj.201695862
Language:
English
Publisher:
EMBO
Publication Date:
2017
detail.hit.zdb_id:
1467419-1
detail.hit.zdb_id:
586044-1
SSG:
12
Permalink