In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 2 ( 2023-2-13), p. e0281770-
Abstract:
A long-term high-fat diet (HFD) causes obesity and changes in renal lipid metabolism and lysosomal dysfunction in mice, causing renal damage. Sodium-glucose co-transporter inhibitors, including phlorizin, exert nephroprotective effects in patients with chronic kidney disease, but the underlying mechanism remains unclear. A HFD or standard diet was fed to adult C57BL/6J male mice, and phlorizin was administered. Lamellar body components of the proximal tubular epithelial cells (PTECs) were investigated. After phlorizin administration in HFD-fed mice, sphingomyelin and ceramide in urine and tissues were assessed and label-free quantitative proteomics was performed using kidney tissue samples. Mitochondrial elongation by fusion was effective in the PTECs of HFD-fed obese mice under phlorizin administration, and many lamellar bodies were found in the apical portion of the S2 segment of the proximal tubule. Phlorizin functioned as a diuretic, releasing lamellar bodies from the apical membrane of PTECs and clearing the obstruction in nephrons. The main component of the lamellar bodies was sphingomyelin. On the first day of phlorizin administration in HFD-fed obese mice, the diuretic effect was increased, and more sphingomyelin was excreted through urine than in vehicle-treated mice. The expressions of three peroxisomal β-oxidation proteins involved in fatty acid metabolism were downregulated after phlorizin administration in the kidneys of HFD-fed mice. Fatty acid elongation protein levels increased with phlorizin administration, indicating an increase in long-chain fatty acids. Lamellar bodies accumulated in the proximal renal tubule of the S2 segment of the HFD-fed mice, indicating that the urinary excretion of lamellar bodies has nephroprotective effects.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0281770
DOI:
10.1371/journal.pone.0281770.g001
DOI:
10.1371/journal.pone.0281770.g002
DOI:
10.1371/journal.pone.0281770.g003
DOI:
10.1371/journal.pone.0281770.g004
DOI:
10.1371/journal.pone.0281770.g005
DOI:
10.1371/journal.pone.0281770.g006
DOI:
10.1371/journal.pone.0281770.g007
DOI:
10.1371/journal.pone.0281770.g008
DOI:
10.1371/journal.pone.0281770.t001
DOI:
10.1371/journal.pone.0281770.s001
DOI:
10.1371/journal.pone.0281770.s002
DOI:
10.1371/journal.pone.0281770.r001
DOI:
10.1371/journal.pone.0281770.r002
DOI:
10.1371/journal.pone.0281770.r003
DOI:
10.1371/journal.pone.0281770.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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