In:
HortScience, American Society for Horticultural Science, Vol. 41, No. 4 ( 2006-07), p. 1069B-1069
Abstract:
Traditionally fatty acid composition used to be analysed by GC and the sample preparation consisted of lipid extraction from sample and subsequent methyl esters preparation, which are time-consuming and cumbersome. As an alternative, simultaneous extraction/methylation methods are being developed for rapid and simplified sample preparation. To optimize one-step extraction/methylation method for analysis of fatty acid composition in brown rice and adlay seeds, various factors, such as sample to reaction solution ratio, reaction time and temperature, and shaking intensity, were altered and resultant fatty acid composition data were evaluated in comparison with previous reports. The ratio of sample weight to reaction solution volume was the most critical factor in that higher sample to reaction solution ratio caused overestimation of palmitic acid and linoleic acid composition, resulting in underestimation of oleic acid. Lower reaction temperature also induced overestimation of linoleic acid and underestimation of oleic acid. Reaction duration and the intensity of shaking prior to and during the reaction, however, induced no significant changes in analysis results. In conclusion, the optimum condition for brown rice was mixing 5 grains (about 0.2 g) of brown rice with 680 μL of methylating mixture and 400 μL of heptane, followed by reaction at 80 °C for 2 hours.
Type of Medium:
Online Resource
ISSN:
0018-5345
,
2327-9834
DOI:
10.21273/HORTSCI.41.4.1069B
Language:
Unknown
Publisher:
American Society for Horticultural Science
Publication Date:
2006
detail.hit.zdb_id:
403302-4
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