In:
Journal of Biomedical Nanotechnology, American Scientific Publishers, Vol. 19, No. 6 ( 2023-06-01), p. 1017-1026
Abstract:
Staphylococcus aureus ( S. aureus ), a Gram-positive bacterium, is often found on the skin, nasal passages, and mucous membranes of healthy individuals, where it acts as a commensal pathogen. Therefore, it is crucial to have a rapid and sensitive detection method to reduce
the spread of S. aureus infections effectively. This study developed a reliable and rapid detection protocol to identify S. aureus DNA in skin infections. The assay exhibited no cross-reactivity with four other bacterial strains and demonstrated a sensitivity of 10 2 CFU/mL. When tested on 24 simulated samples, the MB-LAMP assay results were consistent with those of qPCR, but with a significantly reduced testing time of approximately 30 minutes. Furthermore, this detection scheme can also rapidly detect other pathogens such as Salmonella , Monocytogenes ,
and Vibrio parahaemolyticus .
Type of Medium:
Online Resource
ISSN:
1550-7033
DOI:
10.1166/jbn.2023.3593
Language:
English
Publisher:
American Scientific Publishers
Publication Date:
2023
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