In:
Journal of Immunology Research, Hindawi Limited, Vol. 2019 ( 2019-03-31), p. 1-8
Abstract:
Objective . This study is aimed at investigating the association of exhausted CD8 + tumor-infiltrating lymphocytes with clinic-pathological factors. Methods . 133 patients diagnosed with primary invasive ductal breast cancer were recruited into the cross-sectional study consecutively. Immunohistochemistry was used to detect biomarker expression on formalin-fixed and paraffin-embedded sections. Double staining of CD8 and PD-1 was conducted on lymphocytes. Results . The proportion of CD8 + /PD-1 - TILs was 16% among patients with axillary lymph node metastasis, significantly lower than those without metastasis (24%). The expression of CK7, CK20, or Ki-67 was not related with the proportion of phenotypes of CD8/PD-1 TILs. Younger patients had more cell counts of CD8 + /PD-1 - TILs than elderly patients (18/HPF vs. 9/HPF, p 〈 0.05 ). Patients with axillary lymph node metastasis had less CD8 + /PD-1 - TILs than those without metastasis (11/HPF vs. 27/HPF, p 〈 0.05 ). Median counts of CD8 + /PD-1 - TILs among patients with CK20 and E-Cad expression were 33/HPF and 14/HPF, significantly higher than those among patients with negative CK20 (16/HPF) and E-Cad expression (6/HPF). Ki-67 index had a significant correlation with cell counts of CD8 + /PD-1 + TILs and CD8 + /PD-1 - TILs, and the correlation coefficients were 0.19 and 0.21 ( p 〈 0.05 ), respectively. Conclusion . The proportion of CD8 + /PD-1 - TILs was related with metastatic status of the axillary lymph node but cell counts of CD8 + /PD-1 - TILs were related with metastatic status of the axillary lymph node and expression of CK7, CK20, E-Cad, and Ki-67. Absolute cell counts, not proportion of CD8/PD-1 TILs, were more likely to distinguish clinic and pathologic characteristics of breast cancer.
Type of Medium:
Online Resource
ISSN:
2314-8861
,
2314-7156
DOI:
10.1155/2019/8505021
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2019
detail.hit.zdb_id:
2817541-4
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