In:
Journal of Pathogens, Hindawi Limited, Vol. 2017 ( 2017), p. 1-5
Abstract:
Enzymatic modification results in high-level resistance to aminoglycoside (HLAR), which eliminates the synergistic bactericidal effect of combined exposure to a cell wall-active agent and an aminoglycoside. So aim of the study was to determine prevalence of HLAR enterococcal isolate and to study distribution of aminoglycoside modifying enzyme genes in them. A total of 100 nonrepeat isolates of enterococci from various clinical samples were analyzed. As per Clinical and Laboratory Standards Institute guidelines enterococci were screened for HLAR by Kirby-Bauer disc diffusion method. Minimum inhibitory concentration of all isolates for gentamicin and streptomycin was determined by E-test. Multiplex polymerase chain reaction (PCR) was carried out for HLAR enterococcal isolates to identify aminoglycoside modifying enzymes genes responsible for resistance. 60% isolates were found to be high-level gentamicin resistant (HLGR) whereas 45% isolates were found to be high-level streptomycin resistant (HLSR). By multiplex PCR 80% HLGR isolates carried bifunctional aminoglycoside modifying enzyme gene aac(6 ′ )-Ie-aph(2 ′′ )-Ia whereas 18 out of 45 high-level streptomycin resistant, that is, 40%, isolates carried aph(3 ′ )-IIIa. However, aph(2 ′′ )-Ib, aph(2 ′′ )-Ic, aph(2 ′′ )-Id, and ant(4 ′ )-Ia genes which encode other aminoglycosides modifying enzymes were not detected. Bifunctional aminoglycoside modifying enzyme gene aac(6 ′ )-Ie-aph(2 ′′ )-Ia is the predominant gene responsible for HLAR.
Type of Medium:
Online Resource
ISSN:
2090-3057
,
2090-3065
DOI:
10.1155/2017/3256952
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2017
detail.hit.zdb_id:
2662334-1
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