In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 9 ( 2021-9-16), p. e0257464-
Abstract:
Despite the development of effective vaccines against SARS-CoV-2, epidemiological control of the virus is still challenging due to slow vaccine rollouts, incomplete vaccine protection to current and emerging variants, and unwillingness to get vaccinated. Therefore, frequent testing of individuals to identify early SARS-CoV-2 infections, contact-tracing and isolation strategies remain crucial to mitigate viral spread. Here, we describe WHotLAMP, a rapid molecular test to detect SARS-CoV-2 in saliva. WHotLAMP is simple to use, highly sensitive (~4 viral particles per microliter of saliva) and specific, as well as inexpensive, making it ideal for frequent screening. Moreover, WHotLAMP does not require toxic chemicals or specialized equipment and thus can be performed in point-of-care settings, and may also be adapted for resource-limited environments or home use. While applied here to SARS-CoV-2, WHotLAMP can be modified to detect other pathogens, making it adaptable for other diagnostic assays, including for use in future outbreaks.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0257464
DOI:
10.1371/journal.pone.0257464.g001
DOI:
10.1371/journal.pone.0257464.g002
DOI:
10.1371/journal.pone.0257464.g003
DOI:
10.1371/journal.pone.0257464.g004
DOI:
10.1371/journal.pone.0257464.g005
DOI:
10.1371/journal.pone.0257464.g006
DOI:
10.1371/journal.pone.0257464.t001
DOI:
10.1371/journal.pone.0257464.t002
DOI:
10.1371/journal.pone.0257464.s001
DOI:
10.1371/journal.pone.0257464.s002
DOI:
10.1371/journal.pone.0257464.s003
DOI:
10.1371/journal.pone.0257464.s004
DOI:
10.1371/journal.pone.0257464.s005
DOI:
10.1371/journal.pone.0257464.r001
DOI:
10.1371/journal.pone.0257464.r002
DOI:
10.1371/journal.pone.0257464.r003
DOI:
10.1371/journal.pone.0257464.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
Permalink