In:
The Open Microbiology Journal, Bentham Science Publishers Ltd., Vol. 9, No. 1 ( 2015-7-31), p. 26-32
Abstract:
The development of resistance in bacteria against commonly used antibiotics/drugs is of considerable medical
significance. Aim of this study was to determine the microbial load of un-pasteurized packed fruit juices sold in Lahore city and to determine antibacterial activity of five different honey samples against isolated bacteria. Unpasteurized fruit
juice samples (n=60) were collected from street vendors. All the samples were subjected to Total viable count (TVC), Staphylococcal count (SC) and Coliform count (CC). One hundred and ten strains of bacteria were isolated from various
fruit juices and identified on the basis of cultural characters, morphology and biochemical characters. Mean TVCs, SCs and CCs of juices (6.80±1.91, 5.45±1.06 and 3.25±1.25 log10 CFU/ml respectively) were non-significant with standard
permissible limits (p 〈 0.05). Among all the fruit juices, 66.66% of samples had TVC more than 4 log10 CFU/ml, 51.66%
of samples had SC more than 3 log10 CFU/ml and 46.66% of samples had CC more than 2 log10 CFU/ml. Among the bacillus isolates purified, were Bacillus alvei, Bacillus subtilis, Bacillus polymyxa, Pseudomonas aeruginosa, Staphylococcus
aureus, Klebsiella pneumonia, Escherichia coli and Enterobecter . All five different types of honey samples used
in this study showed antibacterial activity against B. alvei, B. polymyxa, B. subtilis and S. aureus and no activity against P.
aeruginosa, K. pneumonia, Enterobecter and E. coli. It is concluded that microbial load in unpasteurized fruit juices is
significantly higher than standard permissible limits which insinuates its possible role in spoilage and food borne illnesses. Periodic monitoring of packed fruit juices should be carried out to make them safe for consumption. Honey can be used as
an alternative for treatment of various infections, especially those caused by antibiotic resistant bacteria.
Type of Medium:
Online Resource
ISSN:
1874-2858
DOI:
10.2174/1874285801509010026
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
2015
detail.hit.zdb_id:
2395971-X
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