In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 80, No. 16_Supplement ( 2020-08-15), p. 759-759
Abstract:
Precision medicine, specifically in medical oncology, has been significantly enabled and the pace accelerated with the advent of liquid biopsy-based diagnostics. While liquid biopsy assays targeting circulating cell-free DNA (cfDNA) provide useful diagnostic information, such as mutational burden, they are limited to a static assessment of the disease state due to cfDNA derivation from dying cells. Assays exploiting circulating extracellular vesicles (EVs) and EV-associated cargo, such as cell-free RNA (cfRNA), provide a comprehensive and dynamic view of the disease as these analytes derive from both living and dying cells. A significant hurdle in making liquid biopsy testing with EVs and cfRNA commonplace is donor specimen integrity. Collected patient blood samples undergo time-dependent degradation leading to increases in disease non-specific EVs and cfRNA thereby compromising the accuracy and reproducibility of analyte measurement. To overcome this vulnerability we have developed the Streck RNA Complete BCT, a first of its kind blood collection tube intended for stabilizing draw-time concentrations of extracellular vesicles and cell-free RNA. Intended uses include direct analysis of EV populations and indirect analysis of cfRNA through RT-PCR or Next-generation Sequencing-based methods. Incorporation of this blood collection tube into the pre-analytical workflow will allow for delayed sample processing without effect on sample integrity or downstream analyte analysis. Citation Format: Nicholas M. George, Lisa Bartron, Nursen Binbuga, Sean Salamifar, Eunju Seong. RNA Complete BCT: A novel blood collection tube targeting circulating RNA and extracellular vesicles [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 759.
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM2020-759
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2020
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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