In:
PROTEOMICS, Wiley, Vol. 14, No. 10 ( 2014-05), p. 1130-1140
Abstract:
Pilot Project #1—the identification and characterization of human histone H 4 proteoforms by top‐down MS —is the first project launched by the C onsortium for T op‐ D own P roteomics ( CTDP ) to refine and validate top‐down MS . Within the initial results from seven participating laboratories, all reported the probability‐based identification of human histone H 4 ( U ni P rot accession P62805) with expectation values ranging from 10 −13 to 10 −105 . Regarding characterization, a total of 74 proteoforms were reported, with 21 done so unambiguously; one new PTM , K79ac, was identified. Inter‐laboratory comparison reveals aspects of the results that are consistent, such as the localization of individual PTM s and binary combinations, while other aspects are more variable, such as the accurate characterization of low‐abundance proteoforms harboring 〉 2 PTM s. An open‐access tool and discussion of proteoform scoring are included, along with a description of general challenges that lie ahead including improved proteoform separations prior to mass spectrometric analysis, better instrumentation performance, and software development.
Type of Medium:
Online Resource
ISSN:
1615-9853
,
1615-9861
DOI:
10.1002/pmic.201300438
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2037674-1
SSG:
12
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