In:
Development, The Company of Biologists, Vol. 110, No. 2 ( 1990-10-01), p. 555-564
Abstract:
Alkaline phosphatase (AP) activity is stage specific in mouse embryos and may be associated with compaction and separation of trophectoderm from inner cell mass in preimplantation development. We previously sequenced a cDNA and two mouse AP genes that could contribute to the AP activity in embryos. Oligonucleotide primers were constructed from the three sequences and used in the reverse transcription-polymerase chain reaction technique to establish that two of the three AP isozymes are transcribed during preimplantation development. The predominant transcript (E-AP) is from a gene highly homologous to the human tissue-specific APs, but different from the mouse intestinal AP. Tissue non-specific (TN) AP also is transcribed, but there is approximately 10 times less TN-AP than E-AP transcript. The TN-AP isozyme is the predominant transcript of 7 to 14 day embryos and primordial germ cells. A switch in predominance from E-AP to TN-AP must occur during early postimplantation development. This study establishes a framework for experiments to determine the functions of the two isozymes during preimplantation development.
Type of Medium:
Online Resource
ISSN:
0950-1991
,
1477-9129
DOI:
10.1242/dev.110.2.555
Language:
English
Publisher:
The Company of Biologists
Publication Date:
1990
detail.hit.zdb_id:
2007916-3
SSG:
12
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