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  • 1
    Online Resource
    Online Resource
    Modification of an in vitro model simulating the whole digestive process to investigate cellular endpoints of chemoprevention
    Cambridge University Press (CUP) ; 2011
    In:  British Journal of Nutrition Vol. 105, No. 5 ( 2011-03-14), p. 678-687
    Details
    In: British Journal of Nutrition, Cambridge University Press (CUP), Vol. 105, No. 5 ( 2011-03-14), p. 678-687
    Abstract: In vitro gut fermentation systems are relevant tools to study health benefits of foodstuffs. Most of them are commonly used to investigate the degradation of nutrients or the development of gut flora. Using these models, strong cytotoxic effects of the resulting samples on cultured cells were observed. Hence, the aim of the present study was to develop a modified in vitro fermentation model that simulates the whole digestive tract and generates fermented samples that are suitable for testing in cell culture experiments. Wholemeal wheat flour (wwf) was digested and fermented in vitro with a fermentation model using different ox gall concentrations (41·6 and 0·6 g/l). The resulting fermentation supernatants (fs) were characterised for metabolites and biological effects in HT29 cells. The fermentation of wwf increased chemopreventive SCFA and decreased carcinogenic deoxycholic acid (DCA). The strong cytotoxic effects of the fs, which were partly due to cholic acid and DCA, were diminished by lowering the ox gall concentration, allowing the use of the samples in cell culture experiments. In conclusion, an in vitro digestion model, which can be used to study the effects of foodstuffs on chemoprevention and gut health in colon cells, is introduced and its physiological relevance is demonstrated.
    Type of Medium: Online Resource
    ISSN: 0007-1145 , 1475-2662
    URL: Article
    DOI: 10.1017/S0007114510004320
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2011
    detail.hit.zdb_id: 2016047-1
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  • 2
    Online Resource
    Online Resource
    Fermented wheat aleurone inhibits growth and induces apoptosis in human HT29 colon adenocarcinoma cells
    Cambridge University Press (CUP) ; 2010
    In:  British Journal of Nutrition Vol. 103, No. 3 ( 2010-02-14), p. 360-369
    Details
    In: British Journal of Nutrition, Cambridge University Press (CUP), Vol. 103, No. 3 ( 2010-02-14), p. 360-369
    Abstract: Fermentation of dietary fibre by the gut microflora may enhance levels of SCFA, which are potentially chemoprotective against colon cancer. Functional food containing wheat aleurone may prevent cancer by influencing cell cycle and cell death. We investigated effects of fermented wheat aleurone on growth and apoptosis of HT29 cells. Wheat aleurone, flour and bran were digested and fermented in vitro . The resulting fermentation supernatants (fs) were analysed for their major metabolites (SCFA, bile acids and ammonia). HT29 cells were treated for 24–72 h with the fs or synthetic mixtures mimicking the fs in SCFA, butyrate or deoxycholic acid (DCA) contents, and the influence on cell growth was determined. Fs aleurone was used to investigate the modulation of apoptosis and cell cycle. The fermented wheat samples contained two- to threefold higher amounts of SCFA than the faeces control (blank), but reduced levels of bile acids and increased concentrations of ammonia. Fs aleurone and flour equally reduced cell growth of HT29 more effectively than the corresponding blank and the SCFA mixtures. The EC 50 (48 h) ranged from 10 % (flour) to 19 % (blank). Markedly after 48 h, fs aleurone (10 %) significantly induced apoptosis and inhibited cell proliferation by arresting the cell cycle in the G0/G1 phase. In conclusion, fermentation of wheat aleurone results in a reduced level of tumour-promoting DCA, but higher levels of potentially chemopreventive SCFA. Fermented wheat aleurone is able to induce apoptosis and to block cell cycle – two essential markers of secondary chemoprevention.
    Type of Medium: Online Resource
    ISSN: 0007-1145 , 1475-2662
    URL: Article
    DOI: 10.1017/S0007114509991899
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2010
    detail.hit.zdb_id: 2016047-1
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  • 3
    Online Resource
    Online Resource
    Fermentation Supernatants of Wheat (Triticum aestivum L.) Aleurone Beneficially Modulate Cancer Progression in Human Colon Cells
    American Chemical Society (ACS) ; 2010
    In:  Journal of Agricultural and Food Chemistry Vol. 58, No. 3 ( 2010-02-10), p. 2001-2007
    Details
    In: Journal of Agricultural and Food Chemistry, American Chemical Society (ACS), Vol. 58, No. 3 ( 2010-02-10), p. 2001-2007
    Type of Medium: Online Resource
    ISSN: 0021-8561 , 1520-5118
    URL: Article
    DOI: 10.1021/jf9032848
    Language: English
    Publisher: American Chemical Society (ACS)
    Publication Date: 2010
    detail.hit.zdb_id: 1483109-0
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  • 4
    Online Resource
    Online Resource
    A ULTRASSONOGRAFIA NO DIAGNÓSTICO DE AFECÇÕES NOS TESTÍCULOS DE OVINOS (Ovis aries)
    EDUFU - Editora da Universidade Federal de Uberlandia ; 2017
    In:  VETERINÁRIA NOTÍCIAS Vol. 23, No. 1 ( 2017-06-30), p. 1-12
    Details
    In: VETERINÁRIA NOTÍCIAS, EDUFU - Editora da Universidade Federal de Uberlandia, Vol. 23, No. 1 ( 2017-06-30), p. 1-12
    Type of Medium: Online Resource
    ISSN: 1983-0777
    URL: Article
    DOI: 10.14393/VTV-v23n1-2017.1
    Language: Unknown
    Publisher: EDUFU - Editora da Universidade Federal de Uberlandia
    Publication Date: 2017
    detail.hit.zdb_id: 2855134-5
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  • 5
    Online Resource
    Online Resource
    Mechanisms of primary cancer prevention by butyrate and other products formed during gut flora-mediated fermentation of dietary fibre
    Elsevier BV ; 2009
    In:  Mutation Research/Reviews in Mutation Research Vol. 682, No. 1 ( 2009-7), p. 39-53
    Details
    In: Mutation Research/Reviews in Mutation Research, Elsevier BV, Vol. 682, No. 1 ( 2009-7), p. 39-53
    Type of Medium: Online Resource
    ISSN: 1383-5742
    URL: Article
    DOI: 10.1016/j.mrrev.2009.04.001
    RVK:
    WA 15000
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2009
    detail.hit.zdb_id: 2210266-8
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  • 6
    Online Resource
    Online Resource
    Effects of synbiotic fermentation products on primary chemoprevention in human colon cells
    Elsevier BV ; 2012
    In:  The Journal of Nutritional Biochemistry Vol. 23, No. 7 ( 2012-07), p. 777-784
    Details
    In: The Journal of Nutritional Biochemistry, Elsevier BV, Vol. 23, No. 7 ( 2012-07), p. 777-784
    Type of Medium: Online Resource
    ISSN: 0955-2863
    URL: Article
    DOI: 10.1016/j.jnutbio.2011.03.022
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2012
    detail.hit.zdb_id: 1483155-7
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  • 7
    Online Resource
    Online Resource
    Different modes of sodium- d -glucose cotransporter-mediated d -glucose uptake regulation in Caco-2 cells
    American Physiological Society ; 2004
    In:  American Journal of Physiology-Cell Physiology Vol. 287, No. 4 ( 2004-10), p. C1041-C1047
    Details
    In: American Journal of Physiology-Cell Physiology, American Physiological Society, Vol. 287, No. 4 ( 2004-10), p. C1041-C1047
    Abstract: We recently reported that a considerable amount of the sodium-d-glucose cotransporter SGLT1 present in Caco-2 cells, a model for human enterocytes, is located in intracellular compartments attached to microtubules (Kipp H, Khoursandi S, Scharlau D, and Kinne RKH. Am J Physiol Cell Physiol 285: C737–C749, 2003). A similar distribution pattern was also observed in enterocytes in thin sections from human jejunum, highlighting the validity of the Caco-2 cell model. Fluorescent surface labeling of live Caco-2 cells revealed that the intracellular compartments containing SGLT1 were accessible by endocytosis. To elucidate the role of endosomal SGLT1 in the regulation of sodium-dependent d-glucose uptake into enterocytes, we compared SGLT1-mediated d-glucose uptake into Caco-2 cells with the subcellular distribution of SGLT1 after challenging the cells with different stimuli. Incubation (90 min) of Caco-2 cells with mastoparan (50 μM), a drug that enhances apical endocytosis, shifted a large amount of SGLT1 from the apical membrane to intracellular sites and significantly reduced sodium-dependent α-[ 14 C]methyl-d-glucose uptake (−60%). We also investigated the effect of altered extracellular d-glucose levels. Cells preincubated (1 h) with d-glucose-free medium exhibited significantly higher sodium-dependent α-[ 14 C]methyl-d-glucose uptake (+45%) than did cells preincubated with high d-glucose medium (100 mM, 1 h). Interestingly, regulation of SGLT1-mediated d-glucose uptake into Caco-2 cells by extracellular d-glucose levels occurred without redistribution of cellular SGLT1. These data suggest that, pharmacologically, d-glucose uptake can be regulated by a shift of SGLT1 between the plasma membrane and the endosomal pool; however, regulation by the physiological substrate d-glucose can be explained only by an alternative mechanism.
    Type of Medium: Online Resource
    ISSN: 0363-6143 , 1522-1563
    URL: Article
    DOI: 10.1152/ajpcell.00197.2004
    Language: English
    Publisher: American Physiological Society
    Publication Date: 2004
    detail.hit.zdb_id: 1477334-X
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  • 8
    Online Resource
    Online Resource
    Fermented wheat aleurone induces enzymes involved in detoxification of carcinogens and in antioxidative defence in human colon cells
    Cambridge University Press (CUP) ; 2010
    In:  British Journal of Nutrition Vol. 104, No. 8 ( 2010-10-28), p. 1101-1111
    Details
    In: British Journal of Nutrition, Cambridge University Press (CUP), Vol. 104, No. 8 ( 2010-10-28), p. 1101-1111
    Abstract: Dietary fibre is fermented by the human gut flora resulting mainly in the formation of SCFA, for example, acetate, propionate and butyrate. SCFA, in particular butyrate, may be important for secondary cancer prevention by inducing apoptosis and inhibiting cell growth of cancer cells, thereby inhibiting the promotion and/or progression of cancer. Furthermore, SCFA could also act on primary cancer prevention by activation of detoxifying and antioxidative enzymes. We investigated the effects of fermented wheat aleurone on the expression of genes involved in stress response and toxicity, activity of drug-metabolising enzymes and anti-genotoxic potential. Aleurone was digested and fermented in vitro to obtain samples that reflect the content of the colon. HT29 cells and colon epithelial stripes were incubated with the resulting fermentation supernatant fractions (fs) and effects on mRNA expression of CAT , GSTP1 and SULT2B1 and enzyme activity of glutathione S -transferase (GST) and catalase (CAT) were measured. Fermented aleurone was also used to study the protection against H 2 O 2 -induced DNA damage in HT29 cells. The fs of aleurone significantly induced the mRNA expression of CAT , GSTP1 and SULT2B1 (HT29) and GSTP1 (epithelial stripes), respectively. The enzyme activities of GST (HT29) and CAT (HT29, epithelial stripes) were also unambiguously increased (1·4- to 3·7-fold) by the fs of aleurone. DNA damage induced by H 2 O 2 was significantly reduced by the fs of aleurone after 48 h, whereupon no difference was observed compared with the faeces control. In conclusion, fermented aleurone is able to act on primary prevention by inducing mRNA expression and the activity of enzymes involved in detoxification of carcinogens and antioxidative defence.
    Type of Medium: Online Resource
    ISSN: 0007-1145 , 1475-2662
    URL: Article
    DOI: 10.1017/S0007114510001881
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2010
    detail.hit.zdb_id: 2016047-1
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  • 9
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    Online Resource
    Fermentation products of inulin-type fructans reduce proliferation and induce apoptosis in human colon tumour cells of different stages of carcinogenesis
    Cambridge University Press (CUP) ; 2009
    In:  British Journal of Nutrition Vol. 102, No. 5 ( 2009-09-14), p. 663-671
    Details
    In: British Journal of Nutrition, Cambridge University Press (CUP), Vol. 102, No. 5 ( 2009-09-14), p. 663-671
    Abstract: Epidemiological evidence suggests that the intake of prebiotic dietary fibres, for example, inulin, protects against colorectal cancer. However, little is known about cellular responses to complex fermentation samples. Therefore, we prepared a fermentation supernatant fraction of inulin and studied biological properties in human colon cell lines, LT97 and HT29 (representing early and late stages of colon cancer). Inulin enriched with oligofructose (Synergy 1) was incubated under anaerobic conditions with faecal inocula and the supernatant fraction was characterised for content of SCFA and secondary bile acid deoxycholic acid (DCA). A Synergy fermentation supernatant fraction (SFS) and a synthetic fermentation mixture (SFM) mimicking the SFS in SCFA and DCA content were used in the concentration range of 1·25–20 % (v/v) for 24–72 h. The effects on cell growth were determined by quantifying DNA. Effects on apoptosis were analysed by measuring poly(ADP-ribose) polymerase (PARP) cleavage using Western blotting. Compared with the faecal blank, produced without the addition of inulin, the SFS resulted in an almost 2·5-fold increase of SCFA and 3·4-fold decrease of DCA. In comparison with HT29 cells, LT97 cells responded more sensitively to the growth-inhibitory activities. Additionally, a significant increase in PARP cleavage was observed in LT97 cells after incubation with the SFS, demonstrating induction of apoptosis. The present results indicate growth-inhibiting and apoptosis-inducing effects of fermentation supernatant fractions of inulin. Moreover, since early adenoma cells were found to be more sensitive, this may have important implications for chemoprevention when translated to the in vivo situation, because survival of early transformed cells could be reduced.
    Type of Medium: Online Resource
    ISSN: 0007-1145 , 1475-2662
    URL: Article
    DOI: 10.1017/S0007114509274770
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2009
    detail.hit.zdb_id: 2016047-1
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  • 10
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    Online Resource
    In vitro fermented nuts exhibit chemopreventive effects in HT29 colon cancer cells
    Cambridge University Press (CUP) ; 2012
    In:  British Journal of Nutrition Vol. 108, No. 7 ( 2012-10-14), p. 1177-1186
    Details
    In: British Journal of Nutrition, Cambridge University Press (CUP), Vol. 108, No. 7 ( 2012-10-14), p. 1177-1186
    Abstract: It is proven that nuts contain essential macro- and micronutrients, e.g. fatty acids, vitamins and dietary fibre (DF). Fermentation of DF by the gut microflora results in the formation of SCFA which are recognised for their chemopreventive potential, especially by influencing cell growth. However, little is known about cellular response to complex fermentation samples of nuts. Therefore, we prepared and analysed (pH, SCFA, bile acids, tocopherol, antioxidant capacity) fermentation supernatant (fs) fractions of nuts (almonds, macadamias, hazelnuts, pistachios, walnuts) after in vitro fermentation and determined their effects on growth of HT29 cells as well as their genotoxic/anti-genotoxic potential. The fermented nut samples contained 2- to 3-fold higher amounts of SCFA than the faeces control, but considerable reduced levels of bile acids. While most of the investigated native nuts comprised relatively high amounts of tocopherol (α-tocopherol in almonds and hazelnuts and γ- and δ-tocopherol in pistachios and walnuts), rather low concentrations were found in the fs. All nut extracts and nut fs showed a strong antioxidant potential. Furthermore, all fs, except the fs pistachio, reduced growth of HT29 cells significantly. DNA damage induced by H 2 O 2 was significantly reduced by the fs of walnuts after 15 min co-incubation of HT29 cells. In conclusion, this is the first study which presents the chemopreventive effects (reduction of tumour-promoting desoxycholic acid, rise in chemopreventive SCFA, protection against oxidative stress) of different nuts after in vitro digestion and fermentation, and shows the potential importance of nuts in the prevention of colon cancer.
    Type of Medium: Online Resource
    ISSN: 0007-1145 , 1475-2662
    URL: Article
    DOI: 10.1017/S0007114511006647
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2012
    detail.hit.zdb_id: 2016047-1
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    SSG: 21
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