In:
Medicine & Science in Sports & Exercise, Ovid Technologies (Wolters Kluwer Health), Vol. 52, No. 3 ( 2020-3), p. 589-597
Abstract:
DNA methylation may be one of the biological mechanisms underlying the health benefits of physical activity (PA). Our objective was to determine the association between PA and genome-wide DNA methylation at CpG level. Methods We designed a two-stage epigenome wide association study. In the discovery stage, we used 619 individuals from the REgistre GIroní del COR cohort. Next, we validated the CpG suggestively associated with PA ( P 〈 10 −5 ) in two independent populations ( n = 1735 and 190, respectively). Physical activity was assessed with validated questionnaires and classified as light PA (LPA), moderate PA, vigorous PA, moderate-vigorous PA (MVPA) and total PA. We examined linear and nonlinear associations and meta-analyzed the results in the three populations. The linear associations were meta-analyzed with a fixed-effects model and the P values of the nonlinear associations with the Stouffer and Fisher methods. We established a P value threshold that fulfilled Bonferroni criteria over the number of CpG analyzed (0.05/421,940 = 1.185 × 10 −7 ). Results In the meta-analyses, two CpG sites had a statistically significant nonlinear association with MVPA. cg24155427 ( P = 1.19 × 10 −9 ), located in an intergenic region in chromosome 1, has been previously associated with smoking, lupus, and aging. cg09565397 ( P = 1.59 × 10 −7 ), located within DGAT1 in chromosome 8, which encodes an enzyme involved in triacylglycerol synthesis. Conclusions This population-based study identified two new, differentially methylated CpG sites with a nonlinear dose–response relationship to MVPA. These associations must be additionally validated and may be considered for further research on the biological mechanisms underlying health benefits of PA.
Type of Medium:
Online Resource
ISSN:
1530-0315
,
0195-9131
DOI:
10.1249/MSS.0000000000002174
Language:
English
Publisher:
Ovid Technologies (Wolters Kluwer Health)
Publication Date:
2020
detail.hit.zdb_id:
2031167-9
SSG:
31
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