In:
Journal of Separation Science, Wiley, Vol. 44, No. 24 ( 2021-12), p. 4368-4375
Abstract:
Biosynthesis is a promising way to manufacture desired products, however, the purification of its final products is a tough work due to the huge amount of reaction matrix. Liquid stationary phase of high‐speed counter‐current chromatography could easily avoid the commonly disadvantages that occurred in traditional column chromatography in the field of biosynthesized products purification. This characteristic makes high‐speed counter‐current chromatography particularly applicable for final products separation in biosynthesis. In this study, the glycosylation products of Silybin B by one‐pot glycosylation were successfully purified by high‐speed counter‐current chromatography to show the applicability of high‐speed counter‐current chromatography for preparative separation of biosynthesis products. An optimized n ‐hexane/ethyl acetate/methanol/water (2:5:2:3, v/v/v/v ) system was applied in this study. As a result, four Silybin B glycosylation products, including 7 mg of Silybin B‐5‐O‐ β ‐D‐glucoside ( SG‐1 ), 12 mg of Silybin B‐3‐O‐ β ‐D‐glucoside ( SG‐2 ), 10 mg of Silybin B‐7‐O‐ β ‐D‐glucoside ( SG‐3 ), and 24 mg of Silybin B‐20‐O‐ β ‐D‐glucoside ( SG‐4 ), were simultaneously separated from 200 mg of glycosylation crude products, with the purity of 89.3, 95.2, 96.4, and 97.5%, respectively. Their structures were identified by spectroscopic analysis.
Type of Medium:
Online Resource
ISSN:
1615-9306
,
1615-9314
DOI:
10.1002/jssc.202100544
Language:
English
Publisher:
Wiley
Publication Date:
2021
detail.hit.zdb_id:
2047990-6
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