In:
Analytical Cellular Pathology, Hindawi Limited, Vol. 29, No. 3 ( 2007-01-01), p. 249-256
Abstract:
Background : Investigation of the potential association of single nucleotide polymorphisms (SNPs) at –308 G/A and –238 G/A of Tumor necrosis factor α (TNF α ) with susceptibility to HPV-16 associated cervical cancer in Indian women. Methods : The study included 165 histologically confirmed cases with 45 precancer and 120 cancer patients and an equal number (165) of healthy controls with normal cervical cytology. PCR-RFLP was employed to analyze TNF α promoter polymorphisms, which were confirmed by direct sequencing. Both patients and controls were screened for Human Papillomavirus (HPV) infection. Results : The frequency of –308 A allele in TNF α was significantly higher in cases compared with control subjects (21% in cases vs. 9% in controls; p 〈 0.01), with an odds ratio of 2.7 (95% CI = 1.41–5.15). Also, women carrying A allele for this locus presented 3 times increased susceptibility to HPV 16 infection as evident from carrier genotype distribution between HPV positive cases and control subjects (24% in HPV positive cases vs. 9% in controls; p 〈 0.01; OR = 3.1; 95% CI = 1.60–6.03). No such association was found for TNF α –238 (G/A) polymorphism with the risk of development of cervical cancer. Conclusion : It suggests that SNP at –308 (G/A) of TNF α promoter may represent an increased risk for HPV infection and development of cervical cancer in Indian women.
Type of Medium:
Online Resource
ISSN:
2210-7177
,
2210-7185
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2007
detail.hit.zdb_id:
2584078-2
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