In:
Nephron, S. Karger AG, Vol. 86, No. 2 ( 2000), p. 139-144
Abstract:
〈 i 〉 Background/Aims: 〈 /i 〉 In vitro constant calcitriol [1,25-(OH) 〈 sub 〉 2 〈 /sub 〉 D 〈 sub 〉 3 〈 /sub 〉 ] inhibits healthy individuals’ T lymphocyte proliferation at supraphysiological concentrations. In contrast, among hemodialysis patients, intravenous 1,25-(OH) 〈 sub 〉 2 〈 /sub 〉 D 〈 sub 〉 3 〈 /sub 〉 pulse therapy of secondary hyperparathyroidism has been shown to be even immunostimulatory. We studied the effect of in vitro constant and intermittent 1,25-(OH) 〈 sub 〉 2 〈 /sub 〉 D 〈 sub 〉 3 〈 /sub 〉 on lymphocyte antigen response of hemodialysis patients. 〈 i 〉 Methods: 〈 /i 〉 Twelve hemodialysis patients’ peripheral blood mononuclear cells were stimulated with purified protein derivative of tuberculin (12.5, 25 and 50 mg/l) or tetanus toxoid (TT; 1,000, 5,000 and 10,000 Lf/l, limit of flocculation) for 7 days. Constant 1,25-(OH) 〈 sub 〉 2 〈 /sub 〉 D 〈 sub 〉 3 〈 /sub 〉 was added to all cultures at concentrations of 0, 10 〈 sup 〉 –10 〈 /sup 〉 or 0.25 × 10 〈 sup 〉 –9 〈 /sup 〉 mol/l (0, 42 and 105 ng/l) and to half of the cultures additionally as a 0.75 × 10 〈 sup 〉 –9 〈 /sup 〉 mmol/l (315-ng/l) pulse on the 5th culture day. 〈 i 〉 Results: 〈 /i 〉 TT-induced lymphocyte proliferation was statistically related to a constant 1,25-(OH) 〈 sub 〉 2 〈 /sub 〉 D 〈 sub 〉 3 〈 /sub 〉 concentration (p = 0.001, analysis of variance). With constant 1,25-(OH) 〈 sub 〉 2 〈 /sub 〉 D 〈 sub 〉 3 〈 /sub 〉 concentrations of 0, 42 and 105 ng/l, the TT-induced responses were 1.53, 1.44 and 1.40 log cpm, respectively (mean of TT concentrations). The responses of the (additionally) pulse-treated cells [1.65, 1.50 and 1.40 log cpm; concentrations of constant 1,25-(OH) 〈 sub 〉 2 〈 /sub 〉 D 〈 sub 〉 3 〈 /sub 〉 as above] were similar to those of the nonpulsed cells. Thus constant, but not pulsed 1,25-(OH) 〈 sub 〉 2 〈 /sub 〉 D 〈 sub 〉 3 〈 /sub 〉 decreased the TT responses. On the purified protein derivative of tuberculin response, neither constant nor pulsed 1,25-(OH) 〈 sub 〉 2 〈 /sub 〉 D 〈 sub 〉 3 〈 /sub 〉 had any significant effect. 〈 i 〉 Conclusions: 〈 /i 〉 The decline of TT response with constant 1,25-(OH) 〈 sub 〉 2 〈 /sub 〉 D 〈 sub 〉 3 〈 /sub 〉 corresponds with findings on immunosuppressive action of 1,25-(OH) 〈 sub 〉 2 〈 /sub 〉 D 〈 sub 〉 3 〈 /sub 〉 in previous studies done on normal subjects’ cells. This was not seen with intermittently applied 1,25-(OH) 〈 sub 〉 2 〈 /sub 〉 D 〈 sub 〉 3 〈 /sub 〉 . These results support the previous concept that intermittent 1,25(OH) 〈 sub 〉 2 〈 /sub 〉 D 〈 sub 〉 3 〈 /sub 〉 therapy is not immunosuppressive in hemodialysis patients.
Type of Medium:
Online Resource
ISSN:
1660-8151
,
2235-3186
Language:
English
Publisher:
S. Karger AG
Publication Date:
2000
detail.hit.zdb_id:
2810853-X
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