In:
Current Drug Therapy, Bentham Science Publishers Ltd., Vol. 18, No. 5 ( 2023-10), p. 407-414
Abstract:
The use of nanoparticle drug delivery systems to enhance the therapeutic efficacy
and reduce the side effects of anticancer drugs is taken into consideration. Astaxanthin (ATX) is a natural xanthophyll carotenoid with antioxidant, anti-inflammatory, and antiapoptotic properties
used to prevent and treat some cancers. Objectives: In the present study, the antioxidant effect of beta-lactoglobulin (β-LG) nanocapsules containing
ATX and 5-fluorouracil (5-FU; the first-line therapy for colorectal cancer) on the antioxidant enzymes activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX)
in HCT116 colorectal cancer cell line was examined. Methods: In this experimental study, HCT116 cells were treated with different treatments of encapsulation
of ATX in β-LG, encapsulation 5-FU in β-LG, co-encapsulation of ATX and 5-FU in β-LG, free ATX, free 5-FU, free ATX and free 5-FU, or β-LG nanocapsules without drugs for 24, 48 and 72
hours. There is a control group in which HCT116 cells were not treated with any drug. Then, 50% inhibitory concentration (IC50) and cell viability were determined using an MTT assay. The antioxidant
enzyme activity of SOD, CAT, and GPX was measured by a colorimetric method in HCT116 cells. Results: Different treatments reduced the cell viability and increased apoptotic cells in a timedependent
manner, which was significant for beta-lactoglobulin nanocapsules treatment (P 〈 0.05). It
means receiving more 5-FU or ATX in the encapsulated form by HCT116 cells. The antioxidant enzyme activity of SOD, CAT, and GPX in HCT116 cells treated with beta-lactoglobulin nanocapsule
treatment significantly increased compared to the control group (P 〈 0.001). Moreover, the antioxidant
activity of these enzymes in different treatments containing ATX (free or encapsulation) was significantly higher than in other treatments (P 〈 0.05). The most increase in the activity of antioxidant enzymes
is recorded in the treatment of nanocapsules containing ATX and 5-FU simultaneously. Conclusion: Increased activity of antioxidant enzymes in addition to the induction of apoptosis in colorectal
cancer cells by various treatments of beta-lactoglobulin nanocapsules indicates more effective drug administration in encapsulated form as well as synergistic thera[peutic effects of ATX and 5-FU.
Moreover, the main increase in antioxidant enzyme activity may be related to ATX.
Type of Medium:
Online Resource
ISSN:
1574-8855
DOI:
10.2174/1574885518666230403111101
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
2023
SSG:
15,3
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