In:
Journal of Helminthology, Cambridge University Press (CUP), Vol. 65, No. 2 ( 1991-06), p. 149-158
Abstract:
An immunodominant antigen of Dirofilaria immitis was studied using recombinant DNA techniques. The mRNA from D. immitis adult female worms was translated in vitro and a major 34 k Da antigenic polypeptide product was demonstrated by immunoprecipitation. cDNA was synthesized from mRNA and a λ gt11 expression library was constructed and immunoscreened with dirofilariasis positive serum. A positive clone containing a nearly full length cDNA was isolated. The cDNA was 2415 bp in length and consider of a single open reading frame followed by a long 3' non-coding region of 1446 bp. The open reading frame of 969 bp encoded a polypeptide of 322 amino acids with a molecular weight of 34 400. A cDNA fusion protein synthesized by bacteria ( Escherichia coli JM109) using the expression vector pGEMEX-1 was identified as an immunodominant antigen by absorption experiments and had no cross-reactivity with sera from patients with other filarial species.
Type of Medium:
Online Resource
ISSN:
0022-149X
,
1475-2697
DOI:
10.1017/S0022149X00010622
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
1991
detail.hit.zdb_id:
2027960-7
SSG:
12
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