GLORIA

GEOMAR Library Ocean Research Information Access

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    S. Karger AG ; 1984
    In:  International Archives of Allergy and Immunology Vol. 74, No. 1 ( 1984), p. 55-62
    In: International Archives of Allergy and Immunology, S. Karger AG, Vol. 74, No. 1 ( 1984), p. 55-62
    Abstract: The major allergen of birch pollen (BV45) was conjugated to 2–0-methoxy polyethylene glycol-4,6-dichloro-5-triazine (mPEG). Three molecular weight variants of 4,000, 6,000 and 20,000 daltons, respectively, of the activated PEG were used. The modified preparations were labelled by 〈 sup 〉 125 〈 /sup 〉 I and both the native and the radiolabelled protein conjugates were purified by gel filtration chromatography. The relative molecular weights of the purified two peaks were preliminarily estimated by high performance liquid chromatography (two populations of ≥ 100,000 or 20,000 daltons). The amino acid composition of the acid hydrolysates of the three conjugated proteins indicated that 5 residues of lysine were modified by mPEG. Other charged amino acid side chains could also be bound to the activated PEG. The immunochemical properties of the copolymers were studied. The immunogenicity and antigenicity were examined by immunizing rabbits with 〈 sup 〉 125 〈 /sup 〉 I-mPEG 4,000 daltons BV45 and 〈 sup 〉 125 〈 /sup 〉 I-mPEG 20,000 daltons BV45 and subsequently crossed immunoelectrophoresis. The clearence of the radiolabelled protein showed normal pattern. A sharp decline in the radioactivity could be measured. At days 10–12, the remaining radioactivity was below 2%. Preliminary studies showed that the modified proteins were immunogenic in rabbits. The findings were demonstrated by crossed immunoelectrophoresis of the 〈 sup 〉 125 〈 /sup 〉 I-mPEG 4,000, 6,000 or 20,000 daltons BV45 and the corresponding autoradiographs. Apparently, the immunogenicity and antigenicity of the preparations were qualitatively unaltered. The allergenicity of the modified preparation was measured in vitro by RAST and RAST inhibition. The results indicated that the allergenic reactivity of the preparation was reduced by at least 25%. Similar results were shown in vivo by skin prick test (SPT) and PK technique. The reduced allergenic reactivity is likely to be attributed to masking of the immunoreactive determinants of the allergen by attachment to an inert net of polyethylene glycol. These studies showed that conjugation of the major allergen of birch pollen (BV45) resulted in a reduction of the allergenicity. Although the immunogenic and antigenic reactivities seemed qualitatively unaltered, a similar reduction could not be excluded. The data provide an experimental model for the modification of tree pollen allergens, suitable for further application on the manipulation of antibody synthesis in animals.
    Type of Medium: Online Resource
    ISSN: 1018-2438 , 1423-0097
    RVK:
    Language: English
    Publisher: S. Karger AG
    Publication Date: 1984
    detail.hit.zdb_id: 1482722-0
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 2
    Online Resource
    Online Resource
    Wiley ; 1982
    In:  Scandinavian Journal of Immunology Vol. 16, No. 1 ( 1982-07), p. 77-82
    In: Scandinavian Journal of Immunology, Wiley, Vol. 16, No. 1 ( 1982-07), p. 77-82
    Type of Medium: Online Resource
    ISSN: 0300-9475 , 1365-3083
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 1982
    detail.hit.zdb_id: 2020954-X
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 3
    Online Resource
    Online Resource
    International Oil Spill Conference ; 2014
    In:  International Oil Spill Conference Proceedings Vol. 2014, No. 1 ( 2014-05-01), p. 293386-
    In: International Oil Spill Conference Proceedings, International Oil Spill Conference, Vol. 2014, No. 1 ( 2014-05-01), p. 293386-
    Abstract: Statoil is the largest operator on the Norwegian Continental Shelf, is operating 42 assets and has an exploration activity in the order of 20-25 wells per year. In 2011, Statoil decided to undertake a full review of the basis for the oil spill response level for their activities, and establish a fully documented, transparent documentation of their approach. In Norway, operators are required to perform environmental risk and oil spill emergency preparedness assessments for all activities, as a basis for oil spill response plans. These assessments are of a complex and quantitative nature, and it has for a while been realised that there needed to be a better link between well characteristics and the oil spill response level. In the early phase of the development, it was decided to separate issues according to their nature. Issues related to company policy on overall level of spill response were identified and addressed in a separate process, as were issues relating to science and technology. From this, further work proceeded on the three main topics a) Policy decisions on spill response scaling criteria, b) algorithms for calculating effect of various response measures, and c) fact finding on issues of operational windows, capacities and effectiveness. As part of the development, all Company fields in production were reviewed, and oil spill response level adjusted and extended to include near shore and shoreline response plans. Sensitivity studies were undertaken to identify critical elements in the progression from blowout and kill studies to near shore spill response plans. Lessons learned include the overall importance of selecting correct data on reservoir characteristics, sufficient resolution in oil spill modelling, and applying consensus values for spill response capabilities throughout the assessment process. To ensure an adequate basis for near shore spill response planning, GIS technology was applied to produce a set of thematic spill response maps, in A1 size PDF format, for 37 of the most sensitive areas along the Norwegian coast. A strategic plan was also developed for each area. All maps, documentation, GIS data sets and other results of this development work has actively been made available to operators on the NCS, to the Authorities and to spill response organizations.
    Type of Medium: Online Resource
    ISSN: 2169-3358 , 2169-3366
    Language: English
    Publisher: International Oil Spill Conference
    Publication Date: 2014
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 4
    In: Infection and Immunity, American Society for Microbiology, Vol. 67, No. 5 ( 1999-05), p. 2552-2560
    Abstract: Human opsonins directed against specific meningococcal outer membrane structures in sera obtained during meningococcal disease were quantified with a recently developed antigen-specific, opsonin-dependent phagocytosis and oxidative burst assay. Outer membrane vesicles (OMVs) and PorA (class 1) and PorB (class 3) proteins purified from mutants of the same strain (44/76; B:15:P1.7.16) were adsorbed to fluorescent beads, opsonized with acute- and convalescent-phase sera from 40 patients with meningococcal disease, and exposed to human leukocytes. Flow cytometric quantitation of the resulting leukocyte phagocytosis products (PPs) demonstrated that disease-induced serum opsonins recognized meningococcal OMV components and both porins. The PP PorA and PP PorB values induced by convalescent-phase sera correlated positively with the PP OMV values. However, the PP PorB values were higher than the PP PorA values in convalescent-phase sera (medians [ranges] of 754 [17 to 1,057] and 107 [4 to 458], respectively) ( P 〈 0.0001) and correlated positively with higher levels of immunoglobulin G against PorB than against PorA as evaluated by enzyme-linked immunosorbent assay. Extensive individual variations in the anti-OMV and antiporin serum opsonic activities between patients infected by serotypes and serosubtypes homologous and heterologous to the target antigens were observed. Simultaneously measured oxidative burst activity correlated with the opsonophagocytosis, an indication that both of these important steps in the in vitro phagocytic elimination of meningococci are initiated by opsonins directed against OMV components, including PorA and PorB. In conclusion, human patient opsonins against meningococcal OMV components and in particular PorB epitopes were identified by this new method, which might facilitate selection of opsonin-inducing meningococcal antigens for inclusion in future vaccines.
    Type of Medium: Online Resource
    ISSN: 0019-9567 , 1098-5522
    RVK:
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1999
    detail.hit.zdb_id: 1483247-1
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 5
    In: Parasite Immunology, Wiley, Vol. 34, No. 11 ( 2012-11), p. 528-535
    Type of Medium: Online Resource
    ISSN: 0141-9838
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 2020808-X
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 6
    Online Resource
    Online Resource
    American Society for Microbiology ; 1992
    In:  Infection and Immunity Vol. 60, No. 7 ( 1992-07), p. 2777-2783
    In: Infection and Immunity, American Society for Microbiology, Vol. 60, No. 7 ( 1992-07), p. 2777-2783
    Abstract: We have examined the opsonic activity of sera from patients with Neisseria meningitidis (B:15:P1.16) infections against different meningococcal strains, using flow cytometry and luminol-enhanced chemiluminescence. A marked increase in the phagocytosis of ethanol-fixed meningococcal strains of different serogroups, serotypes, and serosubtypes was demonstrated in the presence of convalescence sera compared with acute sera. Convalescence sera also caused a significant increase of leukocyte oxidative metabolism during phagocytosis, as measured by luminol-enhanced chemiluminescence. The sera contained a broad range of opsonins cross-reacting with serogroup A, B, C, W-135, and Y meningococci of different serotypes and serosubtypes, indicating that the cross-reacting opsonins recognized surface epitopes other than those determined by current serotyping schemes.
    Type of Medium: Online Resource
    ISSN: 0019-9567 , 1098-5522
    RVK:
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1992
    detail.hit.zdb_id: 1483247-1
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 7
    In: The Journal of Physical Chemistry A, American Chemical Society (ACS), Vol. 101, No. 48 ( 1997-11-01), p. 8987-8994
    Type of Medium: Online Resource
    ISSN: 1089-5639 , 1520-5215
    RVK:
    Language: English
    Publisher: American Chemical Society (ACS)
    Publication Date: 1997
    detail.hit.zdb_id: 2006031-2
    detail.hit.zdb_id: 1357795-5
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 8
    In: Cell Death & Disease, Springer Science and Business Media LLC, Vol. 7, No. 12 ( 2016-12-01), p. e2497-e2497
    Abstract: Mutated nucleophosmin 1 ( NPM1 ) acts as a proto-oncogene and is present in ~30% of patients with acute myeloid leukemia (AML). Here we examined the in vitro and in vivo anti-leukemic activity of the NPM1 and chromosome region maintenance 1 homolog (CRM1) interacting natural product avrainvillamide (AVA) and a fully syntetic AVA analog. The NPM1 -mutated cell line OCI-AML3 and normal karyotype primary AML cells with NPM1 mutations were significantly more sensitive towards AVA than cells expressing wild-type (wt) NPM1 . Furthermore, the presence of wt p53 sensitized cells toward AVA. Cells exhibiting fms-like tyrosine kinase 3 (FLT3) internal tandem duplication mutations also displayed a trend toward increased sensitivity to AVA. AVA treatment induced nuclear retention of the NPM1 mutant protein (NPMc+) in OCI-AML3 cells and primary AML cells, caused proteasomal degradation of NPMc+ and the nuclear export factor CRM1 and downregulated wt FLT3 protein. In addition, both AVA and its analog induced differentiation of OCI-AML3 cells together with an increased phagocytotic activity and oxidative burst potential. Finally, the AVA analog displayed anti-proliferative activity against subcutaneous xenografted HCT-116 and OCI-AML3 cells in mice. Our results demonstrate that AVA displays enhanced potency against defined subsets of AML cells, suggesting that therapeutic intervention employing AVA or related compounds may be feasible.
    Type of Medium: Online Resource
    ISSN: 2041-4889
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2016
    detail.hit.zdb_id: 2541626-1
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 9
    Online Resource
    Online Resource
    American Society for Microbiology ; 1995
    In:  Infection and Immunity Vol. 63, No. 6 ( 1995-06), p. 2109-2112
    In: Infection and Immunity, American Society for Microbiology, Vol. 63, No. 6 ( 1995-06), p. 2109-2112
    Abstract: Interleukin 10 (IL-10) suppresses the production of proinflammatory cytokines in vitro and in murine models of endotoxemia and has been suggested as a candidate for treatment of bacterial septicemia. To investigate the role of IL-10 in meningococcal disease, a sandwich IL-10 enzyme-amplified sensitivity immunoassay was used to quantitate IL-10 in serum and cerebrospinal fluid samples from 41 patients with meningococcal bacteremia or meningitis with or without septic shock. High levels of IL-10 were demonstrated in sera from patients with meningococcal septic shock (mean, 21,221 pg/ml; range, 25 to 64,500 pg/ml). All cases involving fatalities had IL-10 levels in serum of 〉 or = 1,000 pg/ml (mean, 23,058 pg/ml; range, 1,000 to 64,500 pg/ml). Patients with meningococcal meningitis without septic shock had comparably low concentrations of IL-10 in serum (mean, 119 pg/ml; range, 0 to 1,050 pg/ml) but exhibited compartmentalized release of IL-10 in cerebrospinal fluid. Concentrations of IL-10 in serum were positively correlated with the previously reported concentrations of tumor necrosis factor alpha, IL-6, and IL-8 in serum in the same patients. We conclude that IL-10 is extensively activated along with the proinflammatory cytokines during the initial phase of meningococcal septic shock and that IL-10 is associated with fatality in meningococcal disease.
    Type of Medium: Online Resource
    ISSN: 0019-9567 , 1098-5522
    RVK:
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1995
    detail.hit.zdb_id: 1483247-1
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 10
    Online Resource
    Online Resource
    Elsevier BV ; 1985
    In:  International Journal of Immunopharmacology Vol. 7, No. 1 ( 1985-1), p. 51-55
    In: International Journal of Immunopharmacology, Elsevier BV, Vol. 7, No. 1 ( 1985-1), p. 51-55
    Type of Medium: Online Resource
    ISSN: 0192-0561
    Language: English
    Publisher: Elsevier BV
    Publication Date: 1985
    detail.hit.zdb_id: 1498195-6
    SSG: 15,3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...