In:
Photochemistry and Photobiology, Wiley, Vol. 92, No. 5 ( 2016-09), p. 767-770
Abstract:
A novel conjugate, PHG 10 dye, was synthesized using a collagen peptide and a near–infrared ( NIR )‐responsive dye to achieve targeted cytotoxicity. The collagen peptide motif, ‐(Pro‐Hyp‐Gly) 10 ‐ ( PHG 10), was incorporated for targeting collagen fibrils that are excessively produced by activated fibroblasts around tumor cells. PHG 10 dye was purified by HPLC and identified by MALDI ‐ MS . The phototoxicity and cytotoxicity of PHG 10 dye were examined using human glioma cells ( HGC s). Fluorescent images indicated that PHG 10 dye preferably assembled to collagen‐coated HGC s compared with noncoated HGC s. Under irradiation with NIR light, effective cytotoxicity was observed on collagen‐coated HGC s within 20 min. Because phototoxicity and cytotoxicity are dependent on the assembled amount of PHG 10 dye, the targeting of collagen fibrils by the collagen peptide motif PHG 10 is assured.
Type of Medium:
Online Resource
ISSN:
0031-8655
,
1751-1097
DOI:
10.1111/php.2016.92.issue-5
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2048860-9
SSG:
12
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