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  • 1
    Online Resource
    Online Resource
    PRODUCCIÓN DE BIOGÁS Y CARACTERÍSTICAS FERMENTATIVAS in vitro DE CONSORCIOS BACTERIANOS CELULOLÍTICOS RUMINALES OBTENIDOS DE DIFERENTES FIBRAS
    Universidad Autonoma de Yucatan. Facultad de Medicina Veterinaria y Zootecnia ; 2022
    In:  Tropical and Subtropical Agroecosystems Vol. 25, No. 3 ( 2022-07-12)
    Details
    In: Tropical and Subtropical Agroecosystems, Universidad Autonoma de Yucatan. Facultad de Medicina Veterinaria y Zootecnia, Vol. 25, No. 3 ( 2022-07-12)
    Abstract: 〈 p 〉 〈 strong 〉 Background 〈 /strong 〉 . Cellulolytic bacterial consortia (CBC) serve as additives to improve fiber degradation in ruminants, since they improve biogas production and 〈 em 〉 in vitro 〈 /em 〉 fermentation characteristics. 〈 strong 〉 Objective 〈 /strong 〉 . To evaluate the biogas production and fermentative characteristics 〈 em 〉 in vitro 〈 /em 〉 of mulato grass inoculated with ruminal CBC obtained from ground sawdust, ground mulato grass or whole stem as substrates in the selective culture medium. 〈 strong 〉 Methodology 〈 /strong 〉 . The CBC were obtained from ruminal fluid from a Suiz-Bu cow fitted with a ruminal cannula, which was transferred six times in a selective anaerobic medium. The test consisted of preparing sterile biodigesters with 0.5 g of mulatto grass with 63 d of regrowth, 45 mL of culture medium. The inoculation was with 5 mL of a type of CBC. The biodigesters were incubated 72 h at 39 °C. Biogas production was measured at 3, 6, 9, 12, 24, 48 and 72 h. In the culture media, ammonia nitrogen (N-NH 〈 sub 〉 3 〈 /sub 〉 ), total bacterial count, cellulase activity, pH, dry matter degradation (DMD) and neutral detergent fiber degradation (NDFD) were determined at 12, 24, 48 y 72 h. In the biogas production a completely random design was used; while in the rest of the variables a completely randomized design was carried out with a 3x4 factorial arrangement, with fiber source and incubation time as factors. 〈 strong 〉 Results 〈 /strong 〉 . The biogas production of the CBC obtained from the ground grass showed higher (p & lt;0.05) accumulated production in all the evaluated times. The CBC obtained from sawdust increased the biogas production by 175% from 24 to 48 h and by 313% from 48 to 72 h. There was an interaction effect (p & lt;0.05) on DMD, NDFD, N-NH 〈 sub 〉 3 〈 /sub 〉 , total bacterial count and cellulase enzymatic activity. The CBC obtained from ground mulatto grass at 72 h increased (p & lt;0.05) the DMD and NDFD. The CBC obtained from sawdust increased DMD by 19.1% and NDFD by 33% from 48 to 72 h. The concentration of bacteria in the observed interactions is within the range of the ruminal ecosystem. The highest (p & lt;0.05) content of N-NH 〈 sub 〉 3 〈 /sub 〉 was determined when the CBC obtained from grass stem with 12, 24 and 72 h of incubation were used. 〈 strong 〉 Implications 〈 /strong 〉 . Using the same fiber source to obtain the CBCs improves the degradation of said fiber. 〈 strong 〉 Conclusion 〈 /strong 〉 . The size and source of fiber are decisive in the type of bacteria that make up the cellulolytic bacterial consortia of ruminal origin under the conditions of this 〈 em 〉 in vitro 〈 /em 〉 test. 〈 /p 〉
    Type of Medium: Online Resource
    ISSN: 1870-0462
    URL: Article
    DOI: 10.56369/tsaes.4127
    Language: Unknown
    Publisher: Universidad Autonoma de Yucatan. Facultad de Medicina Veterinaria y Zootecnia
    Publication Date: 2022
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  • 2
    Online Resource
    Online Resource
    Inclusion of ripe mango as a source of energy in diets for Creole lambs in the dry tropics
    Springer Science and Business Media LLC ; 2020
    In:  Tropical Animal Health and Production Vol. 52, No. 6 ( 2020-11), p. 3519-3526
    Details
    In: Tropical Animal Health and Production, Springer Science and Business Media LLC, Vol. 52, No. 6 ( 2020-11), p. 3519-3526
    Type of Medium: Online Resource
    ISSN: 0049-4747 , 1573-7438
    URL: Article
    DOI: 10.1007/s11250-020-02386-4
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2005233-9
    detail.hit.zdb_id: 603363-5
    SSG: 22
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  • 3
    Online Resource
    Online Resource
    Analysis of the Intestinal and Faecal Bacterial Microbiota of the Cervidae Family Using 16S Next-Generation Sequencing: A Review
    MDPI AG ; 2023
    In:  Microorganisms Vol. 11, No. 7 ( 2023-07-24), p. 1860-
    Details
    In: Microorganisms, MDPI AG, Vol. 11, No. 7 ( 2023-07-24), p. 1860-
    Abstract: The Cervidae family has a wide distribution due to its adaptation to numerous ecological environments, which allows it to develop a diverse microbial community in its digestive tract. Recently, research has focused on the taxonomic composition and functionality of the intestinal and faecal microbiota of different cervid species worldwide, as well as their microbial diversity and variation under different associated factors such as age, sex, diet, distribution, and seasonal variation. In addition, there is special interest in knowing how cervids act as reservoirs of zoonotic pathogenic microorganisms, which represent a threat to public health. This review provides a synthesis of the growing field of microbiota determination in cervids worldwide, focusing on intestinal and faecal samples using 16S next-generation sequencing. It also documents factors influencing microbial diversity and composition, the microorganisms reported as pathogenic/zoonotic, and the perspectives regarding the conservation of these species. Knowing the interactions between bacteria and cervid health can drive management and conservation strategies for these species and help develop an understanding of their evolutionary history and the interaction with emerging disease-causing microorganisms.
    Type of Medium: Online Resource
    ISSN: 2076-2607
    URL: Article
    DOI: 10.3390/microorganisms11071860
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2720891-6
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  • 4
    Online Resource
    Online Resource
    In vitro biogas production and degradations of sheep diets containing Crotalaria or Chipile at two different regrowth ages
    Colegio de Postgraduados ; 2022
    In:  Agro Productividad ( 2022-11-16)
    Details
    In: Agro Productividad, Colegio de Postgraduados, ( 2022-11-16)
    Abstract: Objective: To determine the in vitro biogas production and fermentative characteristics of diets for fattening lambs containing 20% chipile or crotalaria at 30 or 40 d of regrowth.Methodology: The treatments were whole diets containing 20% crotalaria with 30 d (T1) or 40 d of regrowth (T2), as well as 20% chipile with 30 d (T3) or 40 d of regrowth (T4). In vitro gas production was measured at 2, 4, 6, 8, 10, 12, 24, 48, and 72 h and the following elements were determined: kinetic estimators (A, b, k), dry matter (DMD), organic matter (OMD), neutral detergent fiber (NDFD), and acid detergent fiber (ADFD) degradation, metabolizable energy (ME), and short-chain fatty acids (SCFA). The experimental design was completely randomized.Results: Regarding the accumulated biogas production, T4 presented higher production from 2 to 24 h, T3 and T4 higher at 48 h, and T1 higher at 72 h. Kinetic estimators showed that T1 was higher in A and k and T4 was higher in b. T2 presented the lowest DMD, OMD, NDFD, ADFD, ME, and SCFA.Study Limitations: Scale production of chipile, aimed to obtain a greater biomass volume, is limited since it has not been domesticated yet. Conclusions: Sheep diets containing 20% chipile or crotalaria with 30 d of regrowth have proven to be an alternative for the manufacturing of whole diets for the intensive fattening of lambs in the tropics.
    Type of Medium: Online Resource
    ISSN: 2594-0252 , 2448-7546
    URL: Article
    DOI: 10.32854/agrop.v15i10.2304
    Language: Unknown
    Publisher: Colegio de Postgraduados
    Publication Date: 2022
    detail.hit.zdb_id: 2926577-0
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  • 5
    Online Resource
    Online Resource
    INULINA: SU USO COMO PREBIÓTICO EN LA NUTRICIÓN DE RUMIANTES
    Universidad Autonoma de Yucatan. Facultad de Medicina Veterinaria y Zootecnia ; 2022
    In:  Tropical and Subtropical Agroecosystems Vol. 25, No. 3 ( 2022-07-12)
    Details
    In: Tropical and Subtropical Agroecosystems, Universidad Autonoma de Yucatan. Facultad de Medicina Veterinaria y Zootecnia, Vol. 25, No. 3 ( 2022-07-12)
    Abstract: 〈 p 〉 〈 strong 〉 Background 〈 /strong 〉 . Prebiotics stimulate the growth and activity of the beneficial microbiota of the digestive tract, improve the health of the host and act as growth promoters. Inulin has prebiotic potential in ruminants, acts as an immunomodulator, improves intestinal health and productive behavior. However, its prebiotic effect on the ruminal microbiota is still unclear, given the little research carried out to date. 〈 strong 〉 Objective 〈 /strong 〉 . This review focuses on the prebiotic effect of inulin and the benefits on the health and productive behavior of ruminants. 〈 strong 〉 Methodology. 〈 /strong 〉 The bibliographic search was carried out in publications of international and national indexed journals, which have a strict peer review, written in English and Spanish, which included information related to inulin, its prebiotic effect, as well as the response of its use in health and ruminant performance. The scientific results of the reviewed articles were compared and contrasted. 〈 strong 〉 Main findings. 〈 /strong 〉 The research analyzed demonstrate the potential use of inulin as a prebiotic in ruminants during lactation and weaning; benefits are shown on intestinal health and reduction in the incidence of diarrhea. In addition, improvements in animal behavior are observed. Inulin improves milk production in cattle. However, the little information available to date does not allow to determine the effect of this prebiotic on the ruminal microbiota. 〈 strong 〉 Implications 〈 /strong 〉 . The information presented in this article shows that the effect of inulin as a prebiotic depends on its degree of polymerization, the age or growth stage of the ruminant, the doses used and, where appropriate, the probiotic population involved. 〈 strong 〉 Conclusions 〈 /strong 〉 . The use of inulin as a prebiotic enhances the probiotic activity of beneficial bacteria, improving intestinal health and ruminant’s performance. However, more research is required on the effect of inulin in the rumen to know its prebiotic potential on the ruminal microbial. 〈 /p 〉
    Type of Medium: Online Resource
    ISSN: 1870-0462
    URL: Article
    DOI: 10.56369/tsaes.4122
    Language: Unknown
    Publisher: Universidad Autonoma de Yucatan. Facultad de Medicina Veterinaria y Zootecnia
    Publication Date: 2022
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  • 6
    Online Resource
    Online Resource
    Effect of waste mango silage on the in vitro gas production, in situ digestibility, intake, apparent digestibility, and ruminal characteristics in calf diets
    Veterinary World ; 2023
    In:  Veterinary World
    Details
    In: Veterinary World, Veterinary World
    Abstract: Background and Aim: Mexico is the fifth largest producer of mangoes in the world. For the conservation of agro-industrial waste and crop residues, the ensiling technique has shown good results. This study aimed to evaluate the effect of increasing the level of mango silage (86% waste mango and 14% pangola grass hay) in calf diets on in vitro gas production, in situ digestibility, intake, apparent digestibility, and ruminal characteristics. Materials and Methods: The diets contained 0 (T0), 30 (T1), 45 (T2), and 60% (T3) mango silage. The partial (24, 48, and 72 h) and cumulative (72 h) biogas, CH4 production, and degradation were determined in the in vitro evaluation. In situ digestibility and estimators of fermentation kinetics of dry matter (DM) and organic matter (OM) were determined. Intake, apparent nutrient digestibility, and rumen parameters of calves (200 kg) were evaluated in a 4 × 4 Latin square design. Response to increased mango silage was calculated by linear and quadratic orthogonal contrasts. Results: In vitro partial and cumulative biogas production decreased linearly (p 〈 0.05), and the partial and cumulative CH4 production did not show linear or quadratic contrast (p 〉 0.05); in vitro DM degradation, in vitro neutral detergent fiber degradation, and in vitro acid detergent fiber degradation showed a linear increase (p 〈 0.05). In situ dry matter digestibility (DMDis), in situ organic matter digestibility (OMDis), b, a + b, c, and effective digestibility (ED) of DMDis, a, a + b, c, and ED of OMDis increased linearly (p 〈 0.05). Dry matter intake, OM intake, and crude protein intake showed a linear increase (p 〈 0.05); NDF intake and ADF intake presented a quadratic behavior (p 〈 0.05). Apparent digestibility of DM, OM, CP, and hemicellulose, pH, N-NH3, total bacterial count, acetate, propionate, butyrate, volatile fatty acids, acetate: propionate ratio, cellulolytic bacteria, and protozoa did not present a linear or quadratic orthogonal effect (p 〉 0.05). Conclusion: The in vitro, in situ, and in vivo variables demonstrated that up to 60% mango silage can be used for the intensive fattening of calves in confinement. Keywords: alternative feed, alternative feeding, cattle, silage, tropics.
    Type of Medium: Online Resource
    ISSN: 2231-0916 , 0972-8988
    URL: Journal
    URL: Article
    DOI: 10.14202/vetworld.
    DOI: 10.14202/vetworld.2023.421-430
    Language: English
    Publisher: Veterinary World
    Publication Date: 2023
    detail.hit.zdb_id: 2420579-5
    detail.hit.zdb_id: 2456277-4
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  • 7
    Online Resource
    Online Resource
    Uso de disacáridos y carbón activado para preservar consorcios de bacterias ruminales celulolíticas liofilizadas
    Universidad de Cordoba ; 2019
    In:  Revista MVZ Córdoba Vol. 24, No. 3 ( 2019-08-28), p. 7305-7313
    Details
    In: Revista MVZ Córdoba, Universidad de Cordoba, Vol. 24, No. 3 ( 2019-08-28), p. 7305-7313
    Abstract: Objetivo. Determinar la fermentación in vitro de consorcios bacterianos ruminales celulolíticos (CBC) conservados por liofilización usando carbón activado, maltosa y lactosa como preservadores. Materiales y métodos. Un CBC se aisló de fluido ruminal de una búfala de agua en medios selectivos celulolíticos. Los CBC se liofilizaron con carbón activado (CA), lactosa (LA) o maltosa (MA) como preservadores y sin preservador (SP). El diseño experimental fue completamente al azar para medir biogás a diferentes intervalos de tiempo; así como, un diseño completamente al azar con arreglo factorial 4x3, los factores fueron preservadores (SP, CA, LA y MA) y tiempo de fermentación (24, 48 y 72 h) para pH, nitrógeno amoniacal (N-NH3), degradación de materia seca (DMS) y de fibra detergente neutro (DFDN), actividad enzimática celulasas y la población de bacterias totales. Resultados. LA produjo mayor biogás acumulado a las 72 h y parcial a partir de las 12 h (p≤0.05). SP no mostró diferencias (p 〉 0.05) en celulasas, conteo de bacterias total, DMS y DFDN en los tiempos de fermentación evaluados con el resto de los preservadores. Conclusiones. La producción de biogás parcial y acumulada, el aumento en la tasa de degradación de 8.3 y 91.1 % en la DMS y DFDN de las 24 a 72 h (p≤0.05) con el preservador LA, muestran que la lactosa puede usarse como preservador de bacterias celulolíticas ruminales.
    Type of Medium: Online Resource
    ISSN: 1909-0544 , 0122-0268
    URL: Issue
    URL: Article
    DOI: 10.21897/rmvz.v24i.n3.2019
    DOI: 10.21897/rmvz.1412
    Language: Unknown
    Publisher: Universidad de Cordoba
    Publication Date: 2019
    detail.hit.zdb_id: 2257624-1
    SSG: 7,36
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  • 8
    Online Resource
    Online Resource
    Productividad de variedades de alfalfa en el Valle de México
    University of Guanajuato ; 2019
    In:  Acta Universitaria Vol. 29 ( 2019-10-02), p. 1-11
    Details
    In: Acta Universitaria, University of Guanajuato, Vol. 29 ( 2019-10-02), p. 1-11
    Abstract: La alfalfa es una de las leguminosas más importantes a nivel mundial y es la más utilizada en la alimentación de ganado lechero en México. El objetivo de la presente investigación consistió en analizar parámetros productivos de variedades de alfalfa comerciales con intervalos de corte definidos estacionalmente, en el Campo Experimental del Colegio de Postgraduados, Montecillo, Estado de México. Las variedades evaluadas fueron Aragón, Valenciana, Chipilo, Milenia y Oaxaca. Las variables evaluadas fueron: tasa de crecimiento, intercepción de luz, índice de área foliar y altura de planta. Milenia y Aragón, con 57 kg DM ha-1 d-1 y 40 kg MS ha-1 d-1, registraron la mayor y menor tasa de crecimiento, respectivamente. La variedad que mayor intercepción de luz registró fue Milenia, con un promedio de 84%. En las cinco variedades de alfalfa, existe una relación positiva entre intercepción de luz, tasa de crecimiento, índice de área foliar y altura de planta. Por sus cualidades de producción se recomienda utilizar la variedad Milenia en el Valle de México.
    Type of Medium: Online Resource
    ISSN: 2007-9621 , 0188-6266
    URL: Article
    DOI: 10.15174/au.2019.2202
    Language: Unknown
    Publisher: University of Guanajuato
    Publication Date: 2019
    detail.hit.zdb_id: 2708030-4
    detail.hit.zdb_id: 2223646-6
    SSG: 7,36
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  • 9
    Online Resource
    Online Resource
    Caracterización y evaluación de frutos de ‘nanche’ (Byrsonima crassifolia L.)
    Revista Mexicana de Ciencias Agricolas ; 2020
    In:  Revista Mexicana de Ciencias Agrícolas Vol. 11, No. 1 ( 2020-02-05), p. 151-160
    Details
    In: Revista Mexicana de Ciencias Agrícolas, Revista Mexicana de Ciencias Agricolas, Vol. 11, No. 1 ( 2020-02-05), p. 151-160
    Abstract: En México, el nanche crece como arbusto en trópico seco y como árbol en zonas de trópico húmedo; produce frutos con aceptación en el mercado, donde cada vez alcanza mayor popularidad. El objetivo de esta investigación fue estudiar las propiedades de calidad físicas y químicas del fruto y las características del endocarpio y embrión del nanche (Byrsonima crassifolia (L.) H. B. K.) colectados en el estado de Oaxaca, México. La investigación se realizó en el Laboratorio de Análisis de Semillas del Colegio de Postgraduados, Campus Montecillo. Se evaluaron cuatro repeticiones de cien frutos, endocarpios y embriones. El análisis de medidas de tendencia central indicó que los frutos tienen forma de oblato, presentan mesocarpio blanco a crema, epicarpio amarillo y sabor característico a la especie. El fruto tiene un endocarpio con cavidades para tres embriones, pero uno o dos se desarrollan, el endocarpio es leñoso, color café oscuro, de forma elíptica y una extremidad acuminada, con surcos sinuosos. El embrión es blanco, ovalado, con cotiledones enrollados en forma de espiral, cubiertos con un tegumento delgado color café. Sin endospermo. Hubo variación en caracteres morfológicos y de calidad.
    Type of Medium: Online Resource
    ISSN: 2007-9230 , 2007-0934
    URL: Article
    DOI: 10.29312/remexca.v11i1.1950
    Language: Unknown
    Publisher: Revista Mexicana de Ciencias Agricolas
    Publication Date: 2020
    detail.hit.zdb_id: 2582680-3
    SSG: 7,36
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  • 10
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    Online Resource
    Fribrinolytic activity and gas production by Pleurotus ostreatus-IE8 and Fomes fomentarius - EUM1 in bagasse cane
    Universidad de Cordoba ; 2015
    In:  Revista MVZ Córdoba Vol. 20, No. supl ( 2015-11-13), p. 4907-4916
    Details
    In: Revista MVZ Córdoba, Universidad de Cordoba, Vol. 20, No. supl ( 2015-11-13), p. 4907-4916
    Abstract: ABSTRACT Objective. To characterize the fibrolytic enzymatic activity of Pleurotus ostreatus-IE8 and Fomes fomentarius-EUM1 in sugarcane bagasse (BCA); to evaluation of the kinetics of in vitro production of BCA treated by solid fermentation (FS), crude enzyme extract (ECE) of P. ostreatus-IE8 and Fibrozyme®. Materials and methods. In fungi measured radial growth rate ( Vcr ) and biomass production in two culture media (with or without nitrogen source); activity of xylanases, cellulases and FS on BCA at 0, 7 and 15 d. The chemical analysis and kinetic analysis of in vitro gas production in 4 treatments (ECE adding enzymes obtained from the direct addition FS or FS ), witness (Fibrozyme®) and a control without addition and analyzed by a was completely randomized design. Results. Xylanases (7 d ) showed 6.32 and 5.50 UI g-1 initial substrate dry weight (SSi) for fungi P. ostreatus-IE8 and F. fomentarius-EUM1 , respectively ; P. ostreatus-IE8 scored higher activity of laccases (10.65 g -1 UI SSi) and F. fomentarius-EUM1 (1.90 UI g-1 SSi) cellulases. The ECE of P. ostreatus-IE8 and commercial enzyme did not differences (p 〉 0.05). In the chemical composition or the gas production kinetics. The 4 treatments evaluated decreased values of the variables measured in the kinetics of gas production compared to the control (p≤0.05). Conclusions. The ECE of P. ostreatus-IE8 was similar to commercial enzyme degradation in vitro, so it is feasible to use pre-digest high fiber products.
    Type of Medium: Online Resource
    ISSN: 1909-0544 , 0122-0268
    URL: Article
    DOI: 10.21897/rmvz.6
    Language: Unknown
    Publisher: Universidad de Cordoba
    Publication Date: 2015
    detail.hit.zdb_id: 2257624-1
    SSG: 7,36
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