In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 18, No. 8 ( 2022-8-25), p. e1010755-
Abstract:
Annual influenza vaccination is recommended to update the variable hemagglutinin antigens. Here, we first designed a virus-like particle (VLP) displaying consensus multi-neuraminidase (NA) subtypes (cN1, cN2, B cNA) and M2 ectodomain (M2e) tandem repeat (m-cNA-M2e VLP). Vaccination of mice with m-cNA-M2e VLP induced broad NA inhibition (NAI), and M2e antibodies as well as interferon-gamma secreting T cell responses. Mice vaccinated with m-cNA-M2e VLP were protected against influenza A (H1N1, H5N1, H3N2, H9N2, H7N9) and influenza B (Yamagata and Victoria lineage) viruses containing substantial antigenic variations. Protective immune contributors include cellular and humoral immunity as well as antibody-dependent cellular cytotoxicity. Furthermore, comparable cross protection by m-cNA-M2e VLP vaccination was induced in aged mice. This study supports a novel strategy of developing a universal vaccine against influenza A and B viruses potentially in both young and aged populations by inducing multi-NA subtype and M2e immunity with a single VLP entity.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1010755
DOI:
10.1371/journal.ppat.1010755.g001
DOI:
10.1371/journal.ppat.1010755.g002
DOI:
10.1371/journal.ppat.1010755.g003
DOI:
10.1371/journal.ppat.1010755.g004
DOI:
10.1371/journal.ppat.1010755.g005
DOI:
10.1371/journal.ppat.1010755.g006
DOI:
10.1371/journal.ppat.1010755.g007
DOI:
10.1371/journal.ppat.1010755.g008
DOI:
10.1371/journal.ppat.1010755.s001
DOI:
10.1371/journal.ppat.1010755.s002
DOI:
10.1371/journal.ppat.1010755.s003
DOI:
10.1371/journal.ppat.1010755.s004
DOI:
10.1371/journal.ppat.1010755.s005
DOI:
10.1371/journal.ppat.1010755.s006
DOI:
10.1371/journal.ppat.1010755.s007
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2205412-1
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