In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 17, No. 6 ( 2023-6-16), p. e0011285-
Abstract:
Salmonella enterica serovar Typhi ( S . Typhi) is the cause of typhoid fever, presenting high rates of morbidity and mortality in low- and middle-income countries. The H58 haplotype shows high levels of antimicrobial resistance (AMR) and is the dominant S . Typhi haplotype in endemic areas of Asia and East sub-Saharan Africa. The situation in Rwanda is currently unknown and therefore to reveal the genetic diversity and AMR of S . Typhi in Rwanda, 25 historical (1984-1985) and 26 recent (2010-2018) isolates from Rwanda were analysed using whole genome sequencing (WGS). WGS was locally implemented using Illumina MiniSeq and web-based analysis tools, thereafter complemented with bioinformatic approaches for more in-depth analyses. Whereas historical S . Typhi isolates were found to be fully susceptible to antimicrobials and show a diversity of genotypes, i.e 2.2.2, 2.5, 3.3.1 and 4.1; the recent isolates showed high AMR rates and were predominantly associated with genotype 4.3.1.2 (H58, 22/26; 84,6%), possibly resulting from a single introduction in Rwanda from South Asia before 2010. We identified practical challenges for the use of WGS in endemic regions, including a high cost for shipment of molecular reagents and lack of high-end computational infrastructure for the analyses, but also identified WGS to be feasible in the studied setting and giving opportunity for synergy with other programs.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0011285
DOI:
10.1371/journal.pntd.0011285.g001
DOI:
10.1371/journal.pntd.0011285.g002
DOI:
10.1371/journal.pntd.0011285.g003
DOI:
10.1371/journal.pntd.0011285.g004
DOI:
10.1371/journal.pntd.0011285.t001
DOI:
10.1371/journal.pntd.0011285.t002
DOI:
10.1371/journal.pntd.0011285.s001
DOI:
10.1371/journal.pntd.0011285.s002
DOI:
10.1371/journal.pntd.0011285.s003
DOI:
10.1371/journal.pntd.0011285.s004
DOI:
10.1371/journal.pntd.0011285.s005
DOI:
10.1371/journal.pntd.0011285.s006
DOI:
10.1371/journal.pntd.0011285.s007
DOI:
10.1371/journal.pntd.0011285.s008
DOI:
10.1371/journal.pntd.0011285.s009
DOI:
10.1371/journal.pntd.0011285.s010
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2429704-5
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