In:
European Journal of Immunology, Wiley, Vol. 42, No. 11 ( 2012-11), p. 2844-2850
Abstract:
Mycobacterium tuberculosis ( MTB )‐specific cytokine responses in the peripheral blood and at the site of infection may differ significantly within the same individual, but the under‐lying T ‐cell subset changes are largely unknown. Here, we measured effector and memory T ‐cell markers on CD 4+ T cells ( CD 45 RO , cysteine chemokine receptor ( CCR )7, and CD 27) in peripheral blood and at the site of active tuberculosis ( TB ). Additionally, T cells were stimulated overnight with purified protein derivative ( PPD ) and early secretory antigenic target ( ESAT )‐6 to determine which T ‐cell subset produces MTB ‐specific interferon ( IFN )‐γ. A striking decrease in CCR 7 and CD 27 expression on T cells was noted at the site of active TB . Likewise, IFN ‐γ expressing, ESAT ‐6 specific CD 4+ CD 45 RO + CD 27− T cells were dramatically increased at the site of infection but were not detectable in peripheral blood. An antigen‐specific expansion of differentiated T cells at the site of active TB infection was poorly reflected in peripheral blood. Insight in these changes in MTB ‐specific effector T cells in different compartments of the body could lead to new approaches for immune‐based diagnosis and interventions.
Type of Medium:
Online Resource
ISSN:
0014-2980
,
1521-4141
DOI:
10.1002/eji.201242678
Language:
English
Publisher:
Wiley
Publication Date:
2012
detail.hit.zdb_id:
1491907-2
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