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  • 1
    Online Resource
    Online Resource
    Tn Red cell polyagglutination in a healthy blood donor: A case report
    Elsevier BV ; 2021
    In:  Transfusion and Apheresis Science Vol. 60, No. 1 ( 2021-02), p. 103013-
    Details
    In: Transfusion and Apheresis Science, Elsevier BV, Vol. 60, No. 1 ( 2021-02), p. 103013-
    Type of Medium: Online Resource
    ISSN: 1473-0502
    URL: Article
    DOI: 10.1016/j.transci.2020.103013
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 2129669-8
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  • 2
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    Analysis of Blood Group Discrepancy in Healthy Blood Donors at a Tertiary Care Referral Hospital from Eastern India: A Retrospective Study
    Georg Thieme Verlag KG ; 2022
    In:  Journal of Laboratory Physicians Vol. 14, No. 03 ( 2022-09), p. 247-252
    Details
    In: Journal of Laboratory Physicians, Georg Thieme Verlag KG, Vol. 14, No. 03 ( 2022-09), p. 247-252
    Abstract: Objective ABO typing constitutes cell grouping and serum grouping. The discrepancy may arise in ABO typing due to a mismatch in cell grouping and serum grouping. It may be due to technical errors, missing or weak ABO antibodies (type I), weak ABO subgroups (type II), Rouleaux formation (type III), or other miscellaneous reasons (type IV). This study was carried out to determine the prevalence and cause of ABO blood group discrepancy in donor samples at our center. Methods A retrospective study of ABO blood group typing of blood donors was conducted at our center. The blood group typing was routinely performed using gel cards and a microcentrifuge system (Tulip Diagnostics(P) Ltd, Goa, India). If any discrepancy in ABO typing was noted, the test was repeated using the conventional tube technique. After sorting clerical/technical error, the causes of discrepancy were analyzed and resolved using anti-A1, anti-H, anti-AB, and other immunohematological tests like antibody screening and identification, saliva inhibition test, adsorption-elution studies. Results A total of 12,715 (98.6% males and 1.4% females) donor samples were tested. The number of ABO discrepancies detected were 15 (0.12%). The discrepancies were characterized as type I (6 cases; 40%), type II (1 case; 6.7%), type III (0 cases; 0%), and type IV (8 cases; 53.3%). Three cases, each of anti-M and anti-Leb, were detected in the study population. A single case of A3, a subgroup of A blood group, was found during the study. Conclusion The prevalence of ABO group discrepancy was 0.12% at our center. Discrepancy arising during ABO typing of blood donor must be resolved before reporting ABO blood group to minimize the recipient's chances of transfusion reaction. The serum grouping is equally crucial as cell grouping for reporting the ABO group of an individual.
    Type of Medium: Online Resource
    ISSN: 0974-2727 , 0974-7826
    URL: Article
    DOI: 10.1055/s-0042-1742417
    Language: English
    Publisher: Georg Thieme Verlag KG
    Publication Date: 2022
    detail.hit.zdb_id: 2461120-7
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  • 3
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    An insight into neonatal unconjugated hyperbilirubinaemia from the transfusion medicine perspective
    Wiley ; 2020
    In:  ISBT Science Series Vol. 15, No. 3 ( 2020-08), p. 325-333
    Details
    In: ISBT Science Series, Wiley, Vol. 15, No. 3 ( 2020-08), p. 325-333
    Abstract: Unconjugated hyperbilirubinaemia is a common cause of morbidity and mortality among neonates in Asian and African countries. The aetiology is multifactorial and has wide range of presentation ranging from simple physiological jaundice to severe HDN requiring prompt intervention to prevent long‐term neurological sequelae. Extensive detection of borderline raised bilirubin level in newborn is debatable as it may lead to inappropriately increased phototherapy and unnecessary increased hospital stay. However, those at risk like early presentation of hyperbilirubinaemia within 48 h, prolonged hyperbilirubinaemia, and family history in siblings or having history of HDN must be screened for immune as well as non‐immune causes to prevent the neonate from further severe form of complications. Along with direct antiglobulin test, elution, antibody screening/identification is performed to evaluate the immune causes. Improvement in the molecular technology leads to prompt diagnosis of non‐immune causes which were previously remained as idiopathic. This will aid in the early management like phototherapy or exchange transfusion as per the indication. This review will basically focus on the aetiopathogenesis of neonatal unconjugated hyperbilirubinaemia and approach to immune causes from transfusion medicine prospective.
    Type of Medium: Online Resource
    ISSN: 1751-2816 , 1751-2824
    URL: Issue
    URL: Article
    DOI: 10.1111/voxs.v15.3
    DOI: 10.1111/voxs.12557
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2250639-1
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  • 4
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    Detection of ABO Discrepancy in a Case of Coronary Artery Disease by Conventional Tube Technique: A Miss by Column Agglutination Technology
    Georg Thieme Verlag KG ; 2022
    In:  Journal of Laboratory Physicians Vol. 14, No. 01 ( 2022-03), p. 087-089
    Details
    In: Journal of Laboratory Physicians, Georg Thieme Verlag KG, Vol. 14, No. 01 ( 2022-03), p. 087-089
    Abstract: ABO and Rh blood grouping of donors and recipients is the first and foremost step in pretransfusion compatibility testing. Conventional tube technique (CTT) is used to test for blood grouping and Rh D typing. But the procedure is cumbersome, and there may be subjective variation during the interpretation of the test results. The other disadvantage is that it is not adaptable to automation. Many newer techniques, such as the column agglutination technique (CAT) used for pretransfusion testing, are amenable to automation. It is being preferred to shift from CTT to semiautomated or fully automated CAT platforms or other newer technologies in many blood centers. The CAT has the added advantage of increased sensitivity and stable end-point results. The results in automated platforms using CAT are equally efficient and reliable as CTT. However, sometimes it is noted that CAT misses subgroups detection. Here, we report a case with a subgroup of A that was failed to be detected by the CAT using dextran acrylamide gel, signifying the use of CTT in evaluating blood group discrepancy.
    Type of Medium: Online Resource
    ISSN: 0974-2727 , 0974-7826
    URL: Article
    DOI: 10.1055/s-0041-1741440
    Language: English
    Publisher: Georg Thieme Verlag KG
    Publication Date: 2022
    detail.hit.zdb_id: 2461120-7
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  • 5
    Online Resource
    Online Resource
    Minor Cross-Matching in the Diagnosis of Pneumococcal Hemolytic Uremic Syndrome in an 18-Month-Old Boy
    Oxford University Press (OUP) ; 2023
    In:  Laboratory Medicine Vol. 54, No. 2 ( 2023-03-07), p. 215-219
    Details
    In: Laboratory Medicine, Oxford University Press (OUP), Vol. 54, No. 2 ( 2023-03-07), p. 215-219
    Abstract: In developing nations, limitations in diagnostic facilities act as a barrier for differentiation of hemolytic uremic syndrome (HUS) based on the etiology. A sick-looking 18-month-old boy presented to our hospital in Bhubaneswar, India, with clinical signs and symptoms of left lobar pneumonia, abnormal hematological and renal parameters, no growth in blood culture, a negative direct antiglobulin test (DAT) result, and low complement levels. A rapid deterioration in his clinical condition necessitated intensive care support, blood transfusion, and renal replacement therapy (peritoneal dialysis and hemodialysis). Because his health care team suspected atypical HUS, therapeutic plasma exchange (TPE) was initiated as soon as possible. In the absence of a lectin panel, minor cross-matching confirmed T-antigen exposure. With a diagnosis of HUS induced by Streptococcus pneumoniae (sp-HUS), TPE was stopped immediately, and washed blood components were administered. Despite the aforementioned measures, the boy died of HUS on day 20 after presentation. This case emphasized the role of minor cross-matching in the detecting of polyagglutination in resolving the diagnostic dilemma of sp-HUS.
    Type of Medium: Online Resource
    ISSN: 0007-5027 , 1943-7730
    URL: Article
    DOI: 10.1093/labmed/lmac092
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2023
    detail.hit.zdb_id: 2100869-3
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  • 6
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    Manual RBC exchange transfusion in a patient of sickle-beta thalassemia syndrome presenting in crisis: a case report
    Medip Academy ; 2017
    In:  International Journal of Advances in Medicine Vol. 4, No. 3 ( 2017-05-23), p. 858-
    Details
    In: International Journal of Advances in Medicine, Medip Academy, Vol. 4, No. 3 ( 2017-05-23), p. 858-
    Abstract: Sickle cell disease and beta thalassemia are caused by abnormal haemoglobin (Hb) derived from mutation of the HBB gene encoding beta-globin. Compound heterozygous status for both mutations results in HbS/beta thalassemia (Sickle- beta thalassemia). Vaso-occlusive phenomena and haemolysis are the clinical hallmarks and major causes of mortality. Here we report a case of successful reduction of HbS level by manual RBC exchange transfusion. Capillary zone electrophoresis showed the case to be Sickle-beta thalassemia. A total of   3 units of 450ml whole blood units were used for manual exchange transfusion done in 2 sittings on consecutive days. Preexchange HbS level was 80.9% of total Hb. HbS level after 24 hours of the second procedure was 44%. In the absence of facility to conduct automated RBC exchange by a cell separator, to reduce HbS in patients presenting with acute complications of SCD and in patients with Vaso-occlusive Crisis, previous stroke, manual RBC exchange can provide a better relief.
    Type of Medium: Online Resource
    ISSN: 2349-3933 , 2349-3925
    URL: Article
    DOI: 10.18203/2349-3933.ijam20172286
    Language: Unknown
    Publisher: Medip Academy
    Publication Date: 2017
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  • 7
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    Hemolytic disease of newborn caused by multiple Rh antibodies
    Medip Academy ; 2019
    In:  International Journal of Contemporary Pediatrics Vol. 6, No. 6 ( 2019-10-21), p. 2688-
    Details
    In: International Journal of Contemporary Pediatrics, Medip Academy, Vol. 6, No. 6 ( 2019-10-21), p. 2688-
    Abstract: Hemolytic disease of Fetus and Newborn (HDFN) usually results due to natural occurring antibodies or alloimmunization in mother but the presence of multiple red cell antibodies increases the risk of development of significant HDFN. Here author reported a case of hemolytic disease of fetus and newborn in a preterm baby caused by multiple maternal antibodies. Direct Antiglobulin Test (DAT) on neonate blood sample was positive (3+) with monospecific DAT showed IgG type which was confirmed by heat elution. Antibody identification of eluate was done using commercial 11-cell panel by gel method showing specificity to anti-D and anti-C antibody which was differentiated from anti-G by sequential adsorption and elution studies. Neonate was treated with double volume exchange transfusion (DVET) using leucoreduced, irradiated O Rh D and C negative PRBC suspended in AB plasma and discharged 6th day in a stable condition. So, all pregnant women should be at least advised for ICT irrespective of Rh D negative status. If ICT is positive, they should be referred to higher center for proper Immunohematological work up, so that proper blood unit for DVET could be identified.
    Type of Medium: Online Resource
    ISSN: 2349-3291 , 2349-3283
    URL: Article
    DOI: 10.18203/2349-3291.ijcp20194755
    Language: Unknown
    Publisher: Medip Academy
    Publication Date: 2019
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  • 8
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    Knowledge, attitudes and practices of resident doctors and interns on safe blood transfusion practices: a survey-based study
    Elsevier BV ; 2023
    In:  Hematology, Transfusion and Cell Therapy Vol. 45, No. 3 ( 2023-07), p. 342-349
    Details
    In: Hematology, Transfusion and Cell Therapy, Elsevier BV, Vol. 45, No. 3 ( 2023-07), p. 342-349
    Type of Medium: Online Resource
    ISSN: 2531-1379
    URL: Article
    DOI: 10.1016/j.htct.2022.06.004
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 2945333-1
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  • 9
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    Impact of COVID ‐19 on blood donor deferral patterns during the COVID ‐19 pandemic: A retrospective analysis
    Wiley ; 2022
    In:  Vox Sanguinis Vol. 117, No. 5 ( 2022-05), p. 656-663
    Details
    In: Vox Sanguinis, Wiley, Vol. 117, No. 5 ( 2022-05), p. 656-663
    Abstract: Blood donor deferral is an essential tool for blood safety. The ongoing COVID‐19 pandemic has adversely affected blood transfusion services all over the world. But its impact on donor deferral rate and the pattern is unclear in light of the new donor deferral policy due to the COVID‐19 pandemic. Materials and Methods This retrospective study was divided into pre‐COVID and COVID (15 March 2019–14 March 2021). All the deferred donors were divided into six different categories: (1) medical causes, (2) surgical causes, (3) drugs and vaccination, (4) risk of transfusion‐transmitted diseases, (5) miscellaneous causes and (6) flu‐like symptoms. In addition, COVID‐related deferrals were also incorporated. All these above categories along with the donor demography were analysed by SPSS software version 25. Results The donor deferral rate was 17.03% and 12.74% during the pre‐COVID and COVID periods, respectively. During the pre‐COVID period, Category 3 deferrals and during COVID period, Category 6 deferrals were significantly higher. A reversal in pattern with increased blood pressure (40.2% vs. 24.04%) over‐riding low haemoglobin (34.77% vs. 55.5%) was noted in the Category 1 deferral during the COVID period. Category 1 deferral was more in middle‐aged adults as compared to young and old adults ( p   〈  0.05). Among middle‐aged adults, deferral due to flu‐like symptoms was also significantly more during the COVID period ( p   〈  0.05). Conclusion COVID‐19 significantly affected the donor pool and changed the pattern of donor deferral. Understanding donor deferral patterns may help in identifying targeted donor populations and planning donor recruitment strategies in future pandemic crises.
    Type of Medium: Online Resource
    ISSN: 0042-9007 , 1423-0410
    URL: Issue
    URL: Article
    DOI: 10.1111/vox.v117.5
    DOI: 10.1111/vox.13240
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 1483587-3
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  • 10
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    Efficacy of therapeutic plasma exchange in severe COVID ‐19 disease: A meta‐analysis
    Wiley ; 2023
    In:  Vox Sanguinis Vol. 118, No. 1 ( 2023-01), p. 49-58
    Details
    In: Vox Sanguinis, Wiley, Vol. 118, No. 1 ( 2023-01), p. 49-58
    Abstract: Therapeutic plasma exchange (TPE) has been used in severe COVID‐19 disease to eliminate the cytokine storm. This meta‐analysis aims to assess the effectiveness of TPE in reducing mortality in severe COVID‐19 disease compared to standard treatment. Materials and Methods A comprehensive literature search was performed in PubMed, the Cochrane database and the International Clinical Trial Registry Platform (ICTRP). The random‐effect model was used to calculate the risk ratio and standardized mean difference (SMD) as pooled effect size for the difference in mortality and length of the intensive care unit (ICU) stay. The risk of bias and publication bias were assessed in R version 4.1.0. The certainty of the evidence was calculated using the GradePro tool. Results The database identified 382 participants from six studies, including one randomized control trial. Egger's test did not detect any publication bias ( p  = 0.178). The random model analysis for mortality evaluated a risk ratio of 0.38 (95% CI: 0.28–0.52) with a significant reduction in the TPE group. The certainty of the evidence was moderate, with a risk ratio of 0.34 (95% CI: 0.24–0.49). Length of ICU stays between TPE versus standard care showed an SMD of 0.08 (95% CI: −0.38, 0.55) and was not significant. Conclusion The length of ICU stay in the TPE group was not different from standard care. However, this meta‐analysis revealed a significant benefit of TPE in reducing mortality in severe COVID‐19 disease compared to standard treatment.
    Type of Medium: Online Resource
    ISSN: 0042-9007 , 1423-0410
    URL: Issue
    URL: Article
    DOI: 10.1111/vox.v118.1
    DOI: 10.1111/vox.13367
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 1483587-3
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