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  • 1
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    Online Resource
    Expression patterns of novel genes during mouse preimplantation embryogenesis
    Wiley ; 1994
    In:  Molecular Reproduction and Development Vol. 37, No. 2 ( 1994-02), p. 121-129
    Details
    In: Molecular Reproduction and Development, Wiley, Vol. 37, No. 2 ( 1994-02), p. 121-129
    Abstract: Little is known about the repertoire of genes expressed following zygotic gene activation, which occurs during the two‐cell stage in the mouse. As an initial attempt to isolate novel genes, we used previously prepared two‐cell and two‐cell subtraction cDNA libraries (Rothstein et al., Genes Dev 6:1190–1201, 1992) to isolate a panel of seven cDNA clones. Three cDNA had no match in the current DNA sequence data banks and three others revealed sequence homology to portions of sequences in the data banks. One cDNA was 90% homologous to the ras ‐related gene Krev / rap 1A. The temporal patterns of expression of these genes during oocyte maturation and preimplantation development were analyzed by a reverse transcription‐polymerase chain reaction (RT‐PCR) assay developed to measure relative levels of mRNAs. Three distinct temporal patterns of expression, designated Classes 1–3, were found. The two Class 1 genes displayed an actin‐like pattern, with a gradual decline in expression during oocyte maturation and through the two‐cell stage, followed by increases at the eight‐cell and/or blastocyst stages. The four genes in Class 2 were expressed at relatively high levels during oocyte maturation and through the one‐cell stage and then declined abruptly between the one‐ and two‐cell stages; an increase then occurred at the eight‐cell and/or blastocyst stages. The expression of the gene in Class 3 declined during oocyte maturation, but then showed a transient increase at the one‐cell stage, with only a very slight increase in synthesis at either the eight‐cell or blastocyst stage. © 1994 Wiley‐Liss, Inc.
    Type of Medium: Online Resource
    ISSN: 1040-452X , 1098-2795
    URL: Issue
    URL: Article
    DOI: 10.1002/mrd.v37:2
    DOI: 10.1002/mrd.1080370202
    Language: English
    Publisher: Wiley
    Publication Date: 1994
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    SSG: 12
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  • 2
    Online Resource
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    Phenotypically diverse mouse thymic stromal cell lines which induce proliferation and differentiation of hematopoietic cells
    Wiley ; 1993
    In:  European Journal of Immunology Vol. 23, No. 6 ( 1993-06), p. 1201-1214
    Details
    In: European Journal of Immunology, Wiley, Vol. 23, No. 6 ( 1993-06), p. 1201-1214
    Abstract: The heterogeneity of the thymic stroma has made careful characterization of particular thymic stromal cell types difficult. To this end, we have derived a panel of cloned thymic stromal cell lines from simian virus 40 T antigen (SV40‐T antigen) transgenic mice. Based on their analysis with monoclonal antibodies that distinguish among subsets of thymic stroma cells, and on the morphology and ultrastructural features of the different clones, we suggest that our panel includes representatives of the thymic subcapsular cortex or thymic nurse cells (427.1), the deep cortex or cortical reticular cells (1308.1) and the medulla including medullary interdigitating (IDC)‐like cells (6.1.1) and medullary epithelial cells (6.1.7). A fifth cell type of undesignated but apparent medullary origin (6.1.11) was also isolated. All of the cell lines constitutively express the SV40 T antigen transgene and the class I antigens of the major histocompatibility complex (MHC), and they can be induced to express MHC class II antigens upon stimulation with recombinant interferon‐γ (IFN‐γ). These cell lines elaborate a factor(s) that induces the proliferation of cells from the fetal liver and bone marrow, but not from the neonatal thymus. A factor(s) elaborated by the 1308.1 cell line also induces the proliferation of fetal thymocytes in the absence of mitogens, phorbol esters or calcium ionophore which is augmented with the addition of recombinant interleukin‐2 (IL‐2). Analysis by reverse transcription polymerase chain reaction with primers for some mouse cytokines reveals that each of these cell lines contain granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) transcripts and that 1308.1,6.1.1 and 6.1.7 produce IL‐6 mRNA. Cell lines 1308.1 and 6.1.1 also produce IL‐7; 6.1.1 produces IL‐lβ and tumor necrosis factor (TNF)‐α while the 427.1 cell line produces IL‐5 and IFN‐γ mRNA. None of the cell lines tested express the IL‐2 receptor, IL‐2, IL‐3, IL‐4, TNF‐β or macrophage inflammatory proteins mRNA. Conditioned medium (CM) from 1308.1 and 6.1.11 induced differentiation of cells purified from the mouse fetal liver into granulocytes; 1308.1 CM also induced differentiation of the mouse hematopoietic stem cell line 32DC13(G) suggesting that the CM contains granulocyte (G)‐CSF activity. Each cell line produces GM‐CSF but the greatest activity is associated with 1308.1 and 6.1.11 CM. The availability of these well‐characterized, functional, cloned thymic stromal cells will allow a more detailed analysis of the role of each cell type in both myeloid and Tcell development.
    Type of Medium: Online Resource
    ISSN: 0014-2980 , 1521-4141
    URL: Issue
    URL: Article
    DOI: 10.1002/eji.v23:6
    DOI: 10.1002/eji.1830230602
    RVK:
    XA 10000
    Language: English
    Publisher: Wiley
    Publication Date: 1993
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  • 3
    Online Resource
    Online Resource
    Expression of the humanAchaete-Scute 1 gene in olfactory neuroblastoma (esthesioneuroblastoma)
    Springer Science and Business Media LLC ; 1995
    In:  Journal of Neuro-Oncology Vol. 26, No. 1 ( 1995-10), p. 35-43
    Details
    In: Journal of Neuro-Oncology, Springer Science and Business Media LLC, Vol. 26, No. 1 ( 1995-10), p. 35-43
    Type of Medium: Online Resource
    ISSN: 0167-594X , 1573-7373
    URL: Article
    DOI: 10.1007/BF01054767
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 1995
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    detail.hit.zdb_id: 2007293-4
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  • 4
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    Knockout of MCT1 results in total absence of spermatozoa, sex hormones dysregulation, and morphological alterations in the testicular tissue
    Springer Science and Business Media LLC ; 2019
    In:  Cell and Tissue Research Vol. 378, No. 2 ( 2019-11), p. 333-339
    Details
    In: Cell and Tissue Research, Springer Science and Business Media LLC, Vol. 378, No. 2 ( 2019-11), p. 333-339
    Type of Medium: Online Resource
    ISSN: 0302-766X , 1432-0878
    URL: Article
    DOI: 10.1007/s00441-019-03028-4
    RVK:
    WA 15000
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2019
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    detail.hit.zdb_id: 125067-X
    SSG: 12
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  • 5
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    Pathologic features of Hashimoto's-associated papillary thyroid carcinomas
    Elsevier BV ; 2001
    In:  Human Pathology Vol. 32, No. 1 ( 2001-1), p. 24-30
    Details
    In: Human Pathology, Elsevier BV, Vol. 32, No. 1 ( 2001-1), p. 24-30
    Type of Medium: Online Resource
    ISSN: 0046-8177
    URL: Article
    DOI: 10.1053/hupa.2001.21138
    RVK:
    XA 10000
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2001
    detail.hit.zdb_id: 207657-3
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  • 6
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    Identification of Functionally Distinct TRAF Proinflammatory and Phosphatidylinositol 3-Kinase/Mitogen-activated Protein Kinase/Extracellular Signal-regulated Kinase Kinase (PI3K/MEK) Transforming Activities Emanating from RET/PTC Fusion Oncoprotein
    Elsevier BV ; 2012
    In:  Journal of Biological Chemistry Vol. 287, No. 6 ( 2012-02), p. 3691-3703
    Details
    In: Journal of Biological Chemistry, Elsevier BV, Vol. 287, No. 6 ( 2012-02), p. 3691-3703
    Type of Medium: Online Resource
    ISSN: 0021-9258
    URL: Article
    DOI: 10.1074/jbc.M111.322677
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2012
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    SSG: 12
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  • 7
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    Cyclooxygenase‐2 Expression in Human Thyroid Carcinoma and Hashimoto's Thyroiditis
    Wiley ; 2002
    In:  The Laryngoscope Vol. 112, No. 2 ( 2002-02), p. 238-242
    Details
    In: The Laryngoscope, Wiley, Vol. 112, No. 2 ( 2002-02), p. 238-242
    Abstract: Objectives Cyclooxygenases (COX) are enzymes that catalyze the conversion of arachidonic acid to prostaglandins. COX‐2, unlike the constitutively expressed COX‐1, is an inducible enzyme upregulated during cell proliferation and inflammation. More recently, COX‐2 has been implicated in the development of numerous types of epithelial cancers. In addition, COX‐2 is highly expressed in several inflammatory diseases. Because of its dual role in inflammation and cancer, we were interested in determining if COX‐2 plays a role in the development of human thyroid carcinoma and Hashimoto's thyroiditis, an autoimmune condition frequently associated with thyroid malignancy. Materials and Methods Twenty paraffin‐embedded human tissue specimens, including normal, inflammatory, and neoplastic thyroid sections, were analyzed by immunohistochemical staining for expression of human COX‐2. In addition, COX‐2 protein expression was verified by Western blot in two specimens. Results Immunohistochemical staining confirmed the presence of COX‐2 in thyroid epithelial neoplasms, including papillary and follicular carcinomas. Moreover, COX‐2 expression was observed in patients with Hashimoto's thyroiditis. COX‐2 expression, however, was not observed in normal thyroid tissue, multinodular goiter, or anaplastic carcinoma. Conclusions We have shown that cyclooxygenase‐2 is expressed in thyroid carcinoma and thyroid epithelium from patients with Hashimoto's thyroiditis but not in normal thyroid. The expression of COX‐2 in both of these thyroid pathologies may provide a basis for the relationship between carcinogenesis and autoimmunity.
    Type of Medium: Online Resource
    ISSN: 0023-852X , 1531-4995
    URL: Article
    DOI: 10.1097/00005537-200202000-00008
    Language: English
    Publisher: Wiley
    Publication Date: 2002
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    detail.hit.zdb_id: 80180-X
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  • 8
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    Inhibition of oncogene-induced inflammatory chemokines using a farnesyltransferase inhibitor
    Springer Science and Business Media LLC ; 2008
    In:  Journal of Inflammation Vol. 5, No. 1 ( 2008-12)
    Details
    In: Journal of Inflammation, Springer Science and Business Media LLC, Vol. 5, No. 1 ( 2008-12)
    Abstract: Farnesyltransferase inhibitors (FTI) are small molecule agents originally formulated to inhibit the oncogenic functions of Ras. Although subsequent analysis of FTI activity revealed wider effects on other pathways, the drug has been demonstrated to reduce Ras signaling by direct measurements. The purpose of the current study was to determine if FTI could be used to inhibit the inflammatory activities of a known Ras-activating human oncoprotein, RET/PTC3. RET/PTC3 is a fusion oncoprotein expressed in the thyroid epithelium of patients afflicted with thyroid autoimmune disease and/or differentiated thyroid carcinoma. Previous studies have demonstrated that RET/PTC3 signals through Ras and can provoke nuclear translocation of NFκB and the downstream release of pro-inflammatory mediators from thyroid follicular cells in vitro and in vivo , making it an ideal target for studies using FTI. Methods For the studies described here, an in vitro assay was developed to measure FTI inhibition of RET/PTC3 pro-inflammatory effects. Rat thyrocytes transfected with RET/PTC3 or vector control cDNA were co-cultured with FTI and examined for inhibition of chemokine expression and secretion measured by RT-PCR and ELISA. Immunoblot analysis was used to confirm the level at which FTI acts on RET/PTC3-expressing cells, and Annexin V/PI staining of cells was used to assess cell death in RET/PTC3-expressing cells co-cultured with FTI. Results These analyses revealed significant mRNA and protein inhibition of chemokines Ccl2 and Cxcl1 with nanomolar doses of FTI. Neither RET/PTC3 protein expression nor apoptosis were affected at any dose of FTI investigated. Conclusion These data suggest that FTI may be applied as an effective inhibitor for RET/PTC3-oncogene induced pro-inflammatory mediators.
    Type of Medium: Online Resource
    ISSN: 1476-9255
    URL: Article
    DOI: 10.1186/1476-9255-5-3
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2008
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  • 9
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    Mucosally Restricted Antigens as Novel Immunological Targets for Antitumor Therapy
    Informa UK Limited ; 2007
    In:  Biomarkers in Medicine Vol. 1, No. 1 ( 2007-06), p. 187-202
    Details
    In: Biomarkers in Medicine, Informa UK Limited, Vol. 1, No. 1 ( 2007-06), p. 187-202
    Type of Medium: Online Resource
    ISSN: 1752-0363 , 1752-0371
    URL: Article
    DOI: 10.2217/17520363.1.1.187
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2007
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    detail.hit.zdb_id: 2491190-2
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  • 10
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    A Thyroid Tumor-Specific Antigen Formed by the Fusion of Two Self Proteins
    The American Association of Immunologists ; 2003
    In:  The Journal of Immunology Vol. 170, No. 2 ( 2003-01-15), p. 861-869
    Details
    In: The Journal of Immunology, The American Association of Immunologists, Vol. 170, No. 2 ( 2003-01-15), p. 861-869
    Abstract: Thyroid epithelial cells frequently express one or more members of the rearranged during transfection/papillary thyroid carcinoma (RET/PTC) fusion oncogene family during early stages of cancer, and fusion gene transcripts have been found in inflammatory conditions of the thyroid such as the autoimmune disease, Hashimoto’s thyroiditis. Because these oncogenes encode chimeric proteins, novel RET/PTC epitopes may be targets of antitumor immune responses. We have been interested in the RET/PTC3 (RP3) fusion protein because this family member is more frequently expressed in radiation-induced and childhood papillary carcinomas than other members of the fusion oncogene family. We hypothesized that the activated kinase of c-RET, in the form of RP3, when expressed in patients with thyroid disease, presents an unusual altered self target for T cell recognition. Interestingly, we find that immunization with mouse RP3 protein can induce a strongly immunogenic response to RP3, although this response is not directed against the peptide comprising the unique fusion region. Rather, the responses are specific for the carboxyl-terminal portion of RP3 that is derived from the self protein c-RET. Furthermore, transplantation of RP3-expressing thyroid tumors into naive mice resulted in leukocytic infiltration, tumor rejection, and induction of RP3-specific T cells. Thus, the somatic fusion of two unrelated self proteins results in the development of a uniquely immunogenic response directed against self epitopes within RP3. These studies may better define the mechanisms controlling the initiation of thyroid-specific immune responses and provide insight into the design of novel molecules for invoking tumor-specific immunity.
    Type of Medium: Online Resource
    ISSN: 0022-1767 , 1550-6606
    URL: Article
    DOI: 10.4049/jimmunol.170.2.861
    RVK:
    XA 54100
    RVK:
    XA 10000
    Language: English
    Publisher: The American Association of Immunologists
    Publication Date: 2003
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