In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 15, No. 6 ( 2021-6-9), p. e0009427-
Abstract:
Chikungunya virus (CHIKV) is an emerging, mosquito-borne alphavirus responsible for acute to chronic arthralgias and neuropathies. Although it originated in central Africa, recent reports of disease have come from many parts of the world, including the Americas. While limiting human CHIKV cases through mosquito control has been used, it has not been entirely successful. There are currently no licensed vaccines or treatments specific for CHIKV disease, thus more work is needed to develop effective countermeasures. Current animal research on CHIKV is often not representative of human disease. Most models use CHIKV needle inoculation via unnatural routes to create immediate viremia and localized clinical signs; these methods neglect the natural route of transmission (the mosquito vector bite) and the associated human immune response. Since mosquito saliva has been shown to have a profound effect on viral pathogenesis, we evaluated a novel model of infection that included the natural vector, Aedes species mosquitoes, transmitting CHIKV to mice containing components of the human immune system. Humanized mice infected by 3–6 mosquito bites showed signs of systemic infection, with demonstrable viremia (by qRT-PCR and immunofluorescent antibody assay), mild to moderate clinical signs (by observation, histology, and immunohistochemistry), and immune responses consistent with human infection (by flow cytometry and IgM ELISA). This model should give a better understanding of human CHIKV disease and allow for more realistic evaluations of mechanisms of pathogenesis, prophylaxis, and treatments.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0009427
DOI:
10.1371/journal.pntd.0009427.g001
DOI:
10.1371/journal.pntd.0009427.g002
DOI:
10.1371/journal.pntd.0009427.g003
DOI:
10.1371/journal.pntd.0009427.g004
DOI:
10.1371/journal.pntd.0009427.g005
DOI:
10.1371/journal.pntd.0009427.g006
DOI:
10.1371/journal.pntd.0009427.g007
DOI:
10.1371/journal.pntd.0009427.g008
DOI:
10.1371/journal.pntd.0009427.t001
DOI:
10.1371/journal.pntd.0009427.t002
DOI:
10.1371/journal.pntd.0009427.t003
DOI:
10.1371/journal.pntd.0009427.s001
DOI:
10.1371/journal.pntd.0009427.s002
DOI:
10.1371/journal.pntd.0009427.s003
DOI:
10.1371/journal.pntd.0009427.s004
DOI:
10.1371/journal.pntd.0009427.s005
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2429704-5
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