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  • 1
    Online Resource
    Online Resource
    Lahore Medical and Dental College ; 2021
    In:  Pakistan Journal of Medical and Health Sciences Vol. 15, No. 10 ( 2021-10-30), p. 2676-2678
    In: Pakistan Journal of Medical and Health Sciences, Lahore Medical and Dental College, Vol. 15, No. 10 ( 2021-10-30), p. 2676-2678
    Abstract: Aim: Helicobacter pylori infection has been ascertained to play pivotal role in the pathogenesis of chronic gastritis and gastric neoplasia.1The present study was performed to evaluate the diagnostic accuracy of H & E stain and Giemsa stain for the histological diagnosis of helicobacter pylori by taking immunohistochemical staining as a gold standard. Methods: A total of 155 cases were included in our study. The received biopsies were fixed in 10% buffered formalin, grossed and stained with H & E and giemsa stain. A board of histopathologists analyzed the morphological details to ascertain the diagnosis. The biopsies were stained by using immunohistochemical techniques against H. pylori antigens, and the procedure was performed according to the guidelines provided by the manufacturer considering the appropriate positive and negative controls for staining. IHC staining was evaluated autonomously and recorded on the proforma as positive and negative cases. Results: In our study, mean age was calculated as 38.4±11.57 years, 74(47.74%) were male and 81(52.26%) were females, frequency of H.Pylori on gold standard was recorded as 109(70.32%), the diagnostic accuracy of hematoxylin-eosin stain for helicobacter pylori detection by taking immunohistochemical staining as a gold standard measure was calculated as 63.30%, 65.22%, 81.18%, 42.86% and 63.87% for sensitivity, specificity, positive predictive value, negative predictive value and accuracy rate respectively, while these findings were recorded as 74.31%, 80.43%, 90%, 56.92% and 76.12% for Giemsa stain. Conclusion: We concluded that the diagnostic accuracy of H & E and Giemsa stains for detection of HP is promising and cost-effective method in our population. MeSH words: Helicobacter pylori, Immunohistochemistry, Hematoxylin, Pathology, Diagnosis
    Type of Medium: Online Resource
    URL: Issue
    Language: Unknown
    Publisher: Lahore Medical and Dental College
    Publication Date: 2021
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  • 2
    In: Cureus, Springer Science and Business Media LLC
    Type of Medium: Online Resource
    ISSN: 2168-8184
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2018
    detail.hit.zdb_id: 2747273-5
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  • 3
    In: Pathology Research International, Hindawi Limited, Vol. 2014 ( 2014-01-06), p. 1-5
    Abstract: The current study was conducted to see the frequency of epithelial malignancies of endometrium with focus on the common diagnostic pitfalls and identify morphological and immunohistochemical markers helpful in the differential diagnosis between different subtypes. It is a retrospective descriptive study carried out on 52 specimens of endometrial tumors received in Fatima Memorial Hospital, Lahore, Pakistan, during three years (2010–2012). Patients were divided into 5 age groups: 〈 40, 41–50, 51–60, 61–70, and 〉 70 yrs. Tissues were fixed in 10% formalin and processed and stained with haematoxylin-eosin. Stained slides were examined to determine the histological types by WHO classification, and immunohistochemistry for WT1, p53, ER/PR, and MIB1 was done in cases where morphology alone was not helpful in making a confirmed diagnosis. 80% of specimens were of endometrioid adenocarcinomas, 11% of serous tumors, 4% of clear cell carcinoma, and 4% of squamous cell carcinomas involving both cervix and endometrium. Most of the patients (28.84%) with endometrial carcinomas fall in the age range of 51–60 yrs. Endometrioid adenocarcinoma is the most common type of epithelial endometrial malignancies. Morphology is the keystone in the evaluation of these tumors, but immunohistochemistry can also be helpful in establishing the correct diagnosis.
    Type of Medium: Online Resource
    ISSN: 2090-8091 , 2042-003X
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2014
    detail.hit.zdb_id: 2573922-0
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  • 4
    Online Resource
    Online Resource
    SAGE Publications ; 2004
    In:  Human & Experimental Toxicology Vol. 23, No. 12 ( 2004-12), p. 547-554
    In: Human & Experimental Toxicology, SAGE Publications, Vol. 23, No. 12 ( 2004-12), p. 547-554
    Abstract: Cisplatin (CDDP) is widely used in the treatment of various cancers but its clinical use is associated with dose limiting nephrotoxicity. The present work was carried out to study the effect of administration of CDDP on rat renal brush border membrane (BBM) marker enzymes and inorganic phosphate (Pi) transport across BBM vesicles (BBMV). Animals were administered a single intraperitoneal dose of CDDP (6 mg/kg body weight) or normal saline and then sacrificed 2, 4, 8 and 16 days after this treatment. The administration of CDDP resulted in increased serum creatinine and blood urea nitrogen levels and decreased activity of BBM marker enzymes in the BBM as well as in the homogenates of cortex and medulla. Kinetic studies showed that the V max of the enzymes was decreased in BBM from CDDP treated rats while the K m remained unchanged. The Na + -gradient dependent transport of Pi across BBMV was also significantly reduced after CDDP treatment. These results strongly suggest that the administration of a single nephrotoxic dose of CDDP results in impairment of the functions of renal BBM.
    Type of Medium: Online Resource
    ISSN: 0960-3271 , 1477-0903
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2004
    detail.hit.zdb_id: 1483723-7
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  • 5
    Online Resource
    Online Resource
    SAGE Publications ; 2007
    In:  Human & Experimental Toxicology Vol. 26, No. 5 ( 2007-05), p. 419-426
    In: Human & Experimental Toxicology, SAGE Publications, Vol. 26, No. 5 ( 2007-05), p. 419-426
    Abstract: Cisplatin is a widely used antineoplastic agent that exhibits dose limiting nephrotoxicity. We have previously shown that the administration of cisplatin results in decrease in the activities of renal brush border membrane (BBM) enzymes and transport of inorganic phosphate (Pi) across BBM vesicles. In the present study we have investigated the effect of pre-treatment with vitamin C (ascorbic acid) on cisplatin-induced nephrotoxicity and changes in BBM enzymes and Pi transport. Administration of a single dose of cisplatin (6 mg/kg body weight) caused nephrotoxicity in rats that manifested biochemically as an elevation of serum urea nitrogen and creatinine levels. Treatment of rats with a single dose of vitamin C, six hours prior to administration of cisplatin, protected the kidney from the damaging effect of cisplatin. Vitamin C pre-treatment significantly decreased the urea nitrogen and creatinine levels. It attenuated the cisplatin-induced reduction in the activities of BBM and anti-oxidant enzymes and also Pi transport. These results suggest that vitamin C is an effective chemoprotectant against cisplatin-induced acute renal failure and dysfunction of the renal BBM in rats. Human & Experimental Toxicology ( 2007) 26, 419—426
    Type of Medium: Online Resource
    ISSN: 0960-3271 , 1477-0903
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2007
    detail.hit.zdb_id: 1483723-7
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  • 6
    In: Current Analytical Chemistry, Bentham Science Publishers Ltd., Vol. 17, No. 1 ( 2020-12-30), p. 49-60
    Abstract: Cadmium contamination is becoming an important issue globally due to its high toxicity and carcinogenicity, leading to detrimental effects on both environmental and human health. This review paper discusses the different sources of cadmium, its toxic effects on the environment and human health, and the influence of different soil factors in changing the speciation and bioavailability of cadmium in soil. This paper also covers research on different remediation strategies currently available for reducing cadmium bioavailability. Methods: Previous research and critical comparison of cadmium extent, toxicity, effects and management were studied Results: The carcinogenic nature of Cadmium is of significant human health concern. Cadmium is found in both free and complexed forms, where its availability dep ends on its speciation in soil. In soil, oxidizing conditions and high pH decrease cadmium bioavailability and speciation. Here, organic matter can also act as both a sink and source of cadmium and microbes perform many mobilization processes including protonation, chelation, and chemical transformation whereas, sorption or precipitation decreases cadmium availability. Continuous exposure to cadmium can result in browning, decomposing and mucilaginous roots, shoot reduction and apical growth of root, chlorosis, and rolling of leaves. Toxicity may inhibit lateral root formation or can result in rigid, twisted, and browning of the main root. Toxicity also affects photosynthesis and the availability of mineral nutrients. In this paper, the remediation techniques of soil polluted by Cd, including biological, chemical and physical remediation are reviewed. The methods of remediation, developing trends, drawbacks, and strengths were also reviewed to provide a reference for the research in this field to date. Conclusion: Although cadmium toxicity is of great concern, many remediation techniques can be successfully used to mitigate its adverse effects. Phytoremediation, hyperaccumulation, bioremediation, and the application of different amendments to the soil have all been successful in alleviating cadmium bioavailability. Amongst all available techniques, in-situ immobilization with inorganic, organic or clay amendments is an environmentally friendly and cost-effective strategy to remediate cadmium contaminated soils and achieve sustainable agriculture production.
    Type of Medium: Online Resource
    ISSN: 1573-4110
    Language: English
    Publisher: Bentham Science Publishers Ltd.
    Publication Date: 2020
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  • 7
    In: Computational and Theoretical Chemistry, Elsevier BV, Vol. 1205 ( 2021-11), p. 113454-
    Type of Medium: Online Resource
    ISSN: 2210-271X
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 2587365-9
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  • 8
    Online Resource
    Online Resource
    Independent Medical Trust ; 2007
    In:  The Professional Medical Journal Vol. 14, No. 04 ( 2007-10-12), p. 648-652
    In: The Professional Medical Journal, Independent Medical Trust, Vol. 14, No. 04 ( 2007-10-12), p. 648-652
    Abstract: Objective: To assess the efficacy of a localization technique for thesentinel lymph node in patients with early breast carcinoma using 1% isosulphan blue. Study Design: Prospective studyPlace and Duration: Departments of Pathology, Punjab Medical College Faisalabad and Sheikh Zayed Hospital,Lahore and Departments of Surgery, Allied & DHQ Hospitals, Faisalabad from July 2002 – June 2003. Patients andMethods: 1% Isosulphan blue was used to identify the sentinel node in cases of early (T1 & T2) breast cancer. Thedye was injected just before the incision was made. The area was massaged. Mastectomy was done. The sentinel nodewas identified by following the blue track to the first coloured lymph node. This was removed and submitted separatelyfor histopathology along with the mastectomy specimen. Results: The procedure was performed in 95 cases. SLNwere identified in 80 (84%) patients. The mean age was 43.99 years. Majority of the tumours were located in the rightbreast in the upper outer quadrant. One SLN was identified in 93%, 2 in 3% and 3 were noted in 4% of the cases.Conclusion: Injection of 1% isosulphan blue around the tumour is an effective method for isolation of the SLN inselected cases. It saves the patient of the side effects of axillary clearance in node negative cases.
    Type of Medium: Online Resource
    ISSN: 2071-7733 , 1024-8919
    Language: Unknown
    Publisher: Independent Medical Trust
    Publication Date: 2007
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  • 9
    In: Journal of Affective Disorders, Elsevier BV, Vol. 349 ( 2024-03), p. 502-508
    Type of Medium: Online Resource
    ISSN: 0165-0327
    RVK:
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2024
    detail.hit.zdb_id: 1500487-9
    SSG: 12
    SSG: 5,2
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  • 10
    Online Resource
    Online Resource
    Elsevier BV ; 1992
    In:  Journal of Food Protection Vol. 55, No. 9 ( 1992-09), p. 708-713
    In: Journal of Food Protection, Elsevier BV, Vol. 55, No. 9 ( 1992-09), p. 708-713
    Type of Medium: Online Resource
    ISSN: 0362-028X
    Language: English
    Publisher: Elsevier BV
    Publication Date: 1992
    detail.hit.zdb_id: 2093078-1
    SSG: 21
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