In:
Bladder, Journal of Biological Methods, Vol. 3, No. 1 ( 2016-02-24), p. e21-
Abstract:
Objective: Single-slice, cross-sectional, intraluminal optical-coherence-tomography (OCT) is a new destruction-free optical imaging method that could be applied clinically to examine the upper urinary tract from within. In-vivo access to the human upper urinary tract (UUT) was attempted. Similar performance statistics as in ex-vivo porcine UUT were expected for delineation of different wall layers by in-vivo OCT of human UUTs.MATERIALS AND METHODS: Institutional ethics committee approved OCT in a volunteer sample of 15 patients suspected of having UUT pathology (urolithiasis, strictures, urothelial carcinoma (UC)). Commercially available, catheter-mounted 400-μm-OCT-probes (lateral resolution, 〈 20 µm) were introduced through a rigid cystoscope and urinary catheter or a rigid ureteroscope. Delineations of different UUT wall layers were recorded for quadrants of OCT images. Continuity-corrected-chi-square-statistics were considered to be significant for P 〈 0.05.RESULTS: OCT was technically successful in all patients. Among quadrants of OCT images of normal UUT in vivo (n = 532) and ex vivo (n = 248), respectively, two observers in consensus found that any tissue layers delineated in 454 (85%) and 223 (90%, P = 0.089), urothelium-lamina propria in 362 (68%) and 193 (78%, P = 0.006), lamina propria-muscle layer in 445 (84%) and 200 (81%, P = 0.352), different muscle layers in 191 (36%) and 207 (84%, P 〈 0.001), and different urothelial cell layers in 17 (3%) and 172 (69%, P 〈 0.001). Histopathology-confirmed non-invasive superficial UC showed intraluminal, papillary urothelial protrusions with undistorted lamina propria in three patients.CONCLUSIONS: In-vivo OCT is technically feasible in the human UUT and may demonstrate urothelium, lamina propria, and muscle layer, and recognize non-invasive UC.
Type of Medium:
Online Resource
ISSN:
2327-2120
DOI:
10.14440/bladder.2016.65
Language:
Unknown
Publisher:
Journal of Biological Methods
Publication Date:
2016
detail.hit.zdb_id:
3035057-8
Permalink