In:
Journal of Experimental Biology, The Company of Biologists, Vol. 207, No. 8 ( 2004-03-15), p. 1323-1334
Abstract:
Cysteine string proteins (CSPs) are conserved secretory vesicle proteins involved in regulating neurotransmitter and peptide release. While the function of the J-domain has been studied in detail, little is known about other conserved regions. We have constructed mutant genes coding for proteins with modified cysteine string, linker region or C terminus and transformed them into Csp null-mutant Drosophila. In the living animal,mutated CSP lacking all cysteines fails to associate with membranes, does not concentrate in synaptic terminals, and cannot rescue adult temperature-sensitive paralysis and short life span, both prominent null mutant phenotypes. A mutant protein with 5 instead of 11 string cysteines appears to be normally targeted but cannot rescue paralysis at 37°C. We propose that the cysteine string, in addition to its role in targeting, may be essential for a function of CSP that is dependent on the number of cysteines in the string. A deletion in the linker region or the C terminus does not affect CSP targeting, and function in adults is only marginally impaired.
Type of Medium:
Online Resource
ISSN:
1477-9145
,
0022-0949
Language:
English
Publisher:
The Company of Biologists
Publication Date:
2004
detail.hit.zdb_id:
1482461-9
SSG:
12
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