In:
Chemistry & Biodiversity, Wiley, Vol. 14, No. 1 ( 2017-01)
Abstract:
This article reports on the in vitro activity of the hydroalcoholic extract of Pfaffia glomerata roots, its hydrolyzed fractions, and pfaffic acid against Trypanosoma cruzi . The hydroalcoholic extract obtained from dried, milled P . glomerata roots was submitted to acid hydrolysis followed by partition with CHC l 3 . The concentrated CHC l 3 fraction was suspended in Me OH /H 2 O and partitioned with hexane ( F1 ), CHC l 3 ( F2 ), and Ac OE t ( F3 ), in this sequence. The trypanocidal activity of the hydrolyzed extract and its fractions was evaluated in vitro . The hydroalcoholic extract displayed low activity, but fraction F1 was active against trypomastigotes of the Y strain of T . cruzi , with IC 50 = 47.89 μg/ml. The steroids campesterol (7.7%), stigmasterol (18.7%), β ‐sitosterol (16.8%), Δ 7 ‐stigmastenol (4.6%), and Δ 7 ‐spinasterol (7.5%) were the major constituents of F1 , along with fatty acid esters (7.6%) and eight aliphatic hydrocarbons (30.1%). Fractions F2 and F3 exhibited moderate activity, and pfaffic acid, one of the main chemical constituents of these fractions, displayed IC 50 = 44.78 μ m (21.06 μg/ml). On the other hand, the hydroalcoholic extract of P . glomerata roots, which is rich in pfaffosides, was inactive. Therefore, the main aglycone of pfaffosides, pfaffic acid, is much more active against trypomastigotes of the Y strain of T . cruzi than its corresponding glycosides and should be further investigated.
Type of Medium:
Online Resource
ISSN:
1612-1872
,
1612-1880
DOI:
10.1002/cbdv.201600175
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2136784-X
detail.hit.zdb_id:
2139001-0
SSG:
12
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