In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 12 ( 2021-12-23), p. e0261076-
Abstract:
MicroRNAs have been proposed as biomarkers for equine sarcoids, the most prevalent equine skin tumors globally. This study served to validate the diagnostic and prognostic potential of whole blood microRNAs identified in a previous study for long-term equine sarcoid diagnosis and outcome prediction. Based on findings of a clinical examination at the age of 3 years and a follow-up following a further 5–12 years, 32 Franches-Montagnes and 45 Swiss Warmblood horses were assigned to four groups: horses with regression (n = 19), progression (n = 9), new occurrences of sarcoid lesions (n = 19) and tumor-free control horses (n = 30). The expression levels for eight microRNAs (eca-miR-127, eca-miR-432, eca-miR-24, eca-miR-125a-5p, eca-miR-134, eca-miR-379, eca-miR-381, eca-miR-382) were analyzed through reverse transcription quantitative polymerase chain reaction in whole blood samples collected on initial examination. Associations of sex, breed, diagnosis, and prognosis with microRNA expression levels were examined using multivariable analysis of variance. Sex and breed influenced the expression level of five and two microRNAs, respectively. Eca-miR-127 allowed discrimination between sarcoid-affected and tumor-free horses. No variation in microRNA expression was found when comparing horses with sarcoid regression and progression. Expression levels of eca-miR-125a-5p and eca-miR-432 varied in male horses that developed sarcoids throughout the study period in comparison to male control horses. While none of the investigated miRNAs was validated for predicting the prognosis of sarcoid regression / progression within young horses with this condition, two miRNAs demonstrated potential to predict if young male (though not female) tumor-free horse can develop sarcoids within the following years. Sex- and breed- biased miRNAs exist within the equine species and have an impact on biomarker discovery.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0261076
DOI:
10.1371/journal.pone.0261076.g001
DOI:
10.1371/journal.pone.0261076.g002
DOI:
10.1371/journal.pone.0261076.g003
DOI:
10.1371/journal.pone.0261076.g004
DOI:
10.1371/journal.pone.0261076.g005
DOI:
10.1371/journal.pone.0261076.t001
DOI:
10.1371/journal.pone.0261076.t002
DOI:
10.1371/journal.pone.0261076.s001
DOI:
10.1371/journal.pone.0261076.s002
DOI:
10.1371/journal.pone.0261076.s003
DOI:
10.1371/journal.pone.0261076.s004
DOI:
10.1371/journal.pone.0261076.s005
DOI:
10.1371/journal.pone.0261076.s006
DOI:
10.1371/journal.pone.0261076.s007
DOI:
10.1371/journal.pone.0261076.s008
DOI:
10.1371/journal.pone.0261076.s009
DOI:
10.1371/journal.pone.0261076.s010
DOI:
10.1371/journal.pone.0261076.r001
DOI:
10.1371/journal.pone.0261076.r002
DOI:
10.1371/journal.pone.0261076.r003
DOI:
10.1371/journal.pone.0261076.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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