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  • 1
    Online Resource
    Online Resource
    Ovid Technologies (Wolters Kluwer Health) ; 2020
    In:  JPO Journal of Prosthetics and Orthotics Vol. 32, No. 4 ( 2020-10), p. 272-285
    In: JPO Journal of Prosthetics and Orthotics, Ovid Technologies (Wolters Kluwer Health), Vol. 32, No. 4 ( 2020-10), p. 272-285
    Abstract: Stroke survivors require physiotherapy and rehabilitation programs to restore their hand function so that they can carry out activities of daily living (ADLs) independently. Soft robotic gloves are designed to assist in these rehabilitation programs and reduce manpower costs, but their pressure-activated actuation mechanisms require closed-loop position feedback to allow for finer motor coordination for the hands, thereby improving hand rehabilitation for patients who had stroke. We present a novel design of graphite-based flex sensors that we implemented in a soft robotic glove to evaluate its performance in closed-loop metacarpophalangeal (MCP) joint angle feedback. Materials and Methods The graphite-based flex sensors are embedded into a sensor glove and characterized in terms of baseline stability and drift over 20 continuous loading cycles per trial for five times. Curve-fitting using both linear and nonlinear equations was done to determine the relationship between resistance and MCP joint angle, using Vicon MX motion capture system to obtain 3D coordinates and joint angles, as well as separate Arduino circuitry to obtain signal voltage samples. Pneumatic pressures are regulated using proportional-integral-derivative (PID) control, with a safety factor (SF) of 1.2. Two control algorithms were developed to make use of angular feedback to control set point pressures: 1) Intent Recognition Mode makes use of a single MCP angle threshold at 50° to activate a maximum output pressure was set at 100 kPa (83.33 kPa after SF); and 2) Fixed Interval Assist Mode makes use of different MCP joint angle values (30°, 45°, 60°, and 90°) to derive corresponding set point pressures set at 25, 50, 75, and 100 kPa (20.83, 41.67, 62.50, and 83.33 kPa after SF). Results Nonlinear equations consistently provided a reasonably better fit as compared with the linear equation fit. However, in this work, the linear MCP joint angle models are preferred as a calibration method, because nonlinear equations are hard to implement in control algorithms in practice. PID control for Intent Recognition activates and deactivates at approximately 18% and 95% of each full flexion-extension exercise cycle progression, respectively. For Fixed Interval Assist Mode, thumb MCP joint angle feedback is less repeatable compared with that of the other fingers in the same experiment, possibly because of the difficulty in placement of the sensor at the thumb MCP joint, close proximity to other sensors, and physiological crosstalk between the fingers. Conclusions This work has presented a novel integration and implementation of graphite-based flex sensors with a soft robotic glove for stroke rehabilitation. The relationship between the signal voltage and the MCP joint angle varies greatly with anatomical differences between each individual, and with sensor placement. However, based on the experimental results, a linear mapping calibration algorithm for the graphite-based flex sensors was implemented, which also complements its robustness for the potential application on stroke rehabilitation. The effectiveness of the calibration algorithm is also thus demonstrated via the Intent Recognition and Fixed Angle Assist control algorithms.
    Type of Medium: Online Resource
    ISSN: 1040-8800
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2020
    detail.hit.zdb_id: 2083552-8
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  • 2
    Online Resource
    Online Resource
    Public Library of Science (PLoS) ; 2016
    In:  PLOS ONE Vol. 11, No. 9 ( 2016-9-9), p. e0162222-
    In: PLOS ONE, Public Library of Science (PLoS), Vol. 11, No. 9 ( 2016-9-9), p. e0162222-
    Type of Medium: Online Resource
    ISSN: 1932-6203
    Language: English
    Publisher: Public Library of Science (PLoS)
    Publication Date: 2016
    detail.hit.zdb_id: 2267670-3
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  • 3
    In: Gastric Cancer, Springer Science and Business Media LLC, Vol. 26, No. 3 ( 2023-05), p. 393-404
    Abstract: We evaluated the relevance of PD-1 + CD8 + T-cells in gastric cancer (GC) including prognostic significance, association with chemotherapy and immunotherapy sensitivity and correlations with the tumor microenvironment (TME). Methods Discovery cohort: GC samples were evaluated for AE1/3, CD8, PD-1, Ki-67 and Granzyme-B expression with fluorescence-based multiplex immunohistochemistry (mIHC). Validation cohorts: we analyzed bulk RNAseq GC datasets from TCGA, the “3G” chemotherapy trial and an immunotherapy phase 2 trial. The cox proportional hazards model was used to identify factors that influenced overall survival (OS). To study the TME, we analyzed single-cell RNAseq performed on GCs. Results In the discovery cohort of 350 GCs, increased PD-1 expression of CD8 T-cells was prognostic for OS (HR 0.822, p  = 0.042). PD-1 expression in CD8 T-cells highly correlated with cytolytic [Granzyme-B + ] ( r  = 0.714, p   〈  0.001) and proliferative [Ki-67 + ] ( r  = 0.798, p   〈  0.001) activity. Analysis of bulk RNAseq datasets showed tumors with high PD-1 and CD8A expression levels had improved OS when treated with immunotherapy (HR 0.117, p  = 0.036) and chemotherapy (HR 0.475, p  = 0.017). Analysis of an scRNAseq dataset of 152,423 cells from 40 GCs revealed that T-cell and NK-cell proportions were higher (24% vs 18% and 19% vs 15%, p   〈  0.0001), while macrophage proportions were lower (7% vs 11%, p   〈  0.0001) in CD8PD-1 high compared to CD8PD-1 low tumors. Conclusion This is one of the largest GC cohorts of mIHC combined with analysis of multiple datasets providing orthogonal validation of the clinical relevance of PD-1 + CD8 + T-cells being associated with improved OS. CD8PD-1 high tumors have distinct features of an immunologically active, T-cell inflamed TME.
    Type of Medium: Online Resource
    ISSN: 1436-3291 , 1436-3305
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 1481763-9
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  • 4
    In: Neurogastroenterology & Motility, Wiley, Vol. 35, No. 7 ( 2023-07)
    Abstract: Irritable bowel syndrome (IBS) is a prevalent gastrointestinal disease characterized by intermittent abdominal pain with altered bowel habits. Due to the condition's chronicity, patients suffer from poor quality of life, while the healthcare burden continues to grow. There is currently no reliable biomarker for the diagnosis of IBS, and the current approach depends on ruling‐out organic diseases such as inflammatory bowel disease (IBD) and colorectal cancer by markers of inflammation like fecal calprotectin and C‐reactive protein, or invasive procedures like a colonoscopy. Volatile organic compounds (VOCs) are growing in popularity as a biomarker due to its accuracy and ease of use. Purpose This systematic review of Medline and Cochrane's databases aimed to identify VOCs in the diagnosis of IBS. 57% of the studies proved that VOCs could identify IBS patients from healthy controls with AUC ranging from 0.83 to 0.99. Studies that distinguished IBS from IBD patients had slightly higher AUC of 0.87–0.98. Combining VOC into panels allowed the creation of discriminative algorithms. Though current research is limited by areas of heterogeneity in VOC sampling and small sample sizes, our review shows that VOC analysis has the potential to be a noninvasive point‐of‐care test that differentiates IBS from other organic gastrointestinal diseases.
    Type of Medium: Online Resource
    ISSN: 1350-1925 , 1365-2982
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2008278-2
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  • 5
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2017
    In:  Biological Research Vol. 50, No. 1 ( 2017-12)
    In: Biological Research, Springer Science and Business Media LLC, Vol. 50, No. 1 ( 2017-12)
    Type of Medium: Online Resource
    ISSN: 0717-6287
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2017
    detail.hit.zdb_id: 2048380-6
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  • 6
    Online Resource
    Online Resource
    Science Trends ; 2018
    In:  Science Trends ( 2018-01-11)
    In: Science Trends, Science Trends, ( 2018-01-11)
    Type of Medium: Online Resource
    Language: English
    Publisher: Science Trends
    Publication Date: 2018
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