In:
Molecular Biology of the Cell, American Society for Cell Biology (ASCB), Vol. 33, No. 14 ( 2022-12-01)
Abstract:
The nuclear pore complex (NPC) is a highly modular assembly of 34 distinct nucleoporins (Nups) to form a versatile transport channel between the nucleus and the cytoplasm. Among them, Nup62 is known as an essential component for nuclear transport, Nup93 for proper nuclear envelope assembly. These Nups constitute various NPC subcomplexes such as the central transport channel (CTC), the cytoplasmic ring (CR), and the inner ring (IR). However, how they play their roles in NPC assembly and transport activity is not clear. Here we delineated the interacting regions and conducted biochemical reconstitution and structural characterization of the mammalian CR complex to reveal its intrinsic dynamic behavior and a distinct “4”-shaped architecture resembling the CTC complex. Our in vitro reconstitution data demonstrate that the Nup62 coiled-coil domain is critical to form both Nup62 322-525 •Nup88 517-742 and Nup62 322-525 •Nup88 517-742 •Nup214 693-926 heterotrimers and both can bind to Nup93 1-150 . We therefore propose that Nup93 acts as a “sensor” to bind to Nup62 shared heterotrimers including the Nup62•Nup54 heterotrimer of the CTC, which was not shown previously to be an interacting partner. Altogether, our biochemical study suggests that Nup62 via its coiled-coil domain is central to form compositionally distinct yet structurally similar heterotrimers and Nup93 binds these diverse heterotrimers nonselectively.
Type of Medium:
Online Resource
ISSN:
1059-1524
,
1939-4586
DOI:
10.1091/mbc.E22-01-0027
Language:
English
Publisher:
American Society for Cell Biology (ASCB)
Publication Date:
2022
detail.hit.zdb_id:
1474922-1
SSG:
12
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