In:
Bulletin of the Veterinary Institute in Pulawy, Walter de Gruyter GmbH, Vol. 56, No. 3 ( 2012-09-01), p. 373-378
Abstract:
The aim of the study was to investigate the effect of 2-methoxyestradiol (2-ME) on GnRH-induced LH and FSH secretion by porcine pituitary cells in vitro . Moreover, the concentrations of superoxide anion radical (O 2 - ), as a possible mediator of 2-ME action, and malonyldialdehyde (MDA), as an oxidative stress indicator, were estimated. Pituitary cells were cultured in McCoy 5A medium with GnRH (positive control), with GnRH and 3.3×10 -11 -3.3×10 -7 M/L of 2-ME, or with GnRH and 2.7×10 -9 - 2.7×10 -8 M/L of 17β-estradiol. The secretion of gonadotropins, as well as concentrations of superoxide anion and MDA were analysed after 2-72 h of the experiment. A positive correlation between 2-ME dose and FSH secretion from anterior pituitary cells (r=0.72, 0.95, 0.92, 0.90, 0.85, and 0.82 after 2, 6, 18, 24, 48, and 72 h, respectively) was demonstrated. In contrast, a negative correlation between 2-ME concentration and LH secretion was observed (r=-0.82, -0.72, -0.85, -0.89, -0.93, and -0.79 after 2, 6, 18, 24, 48, and 72 h, respectively). The concentration of O 2 - . was increasing under the influence of the rising doses of 2-ME, whereas the mean level of MDA was not changing significantly.
Type of Medium:
Online Resource
ISSN:
2300-3235
DOI:
10.2478/v10213-012-0065-6
Language:
English
Publisher:
Walter de Gruyter GmbH
Publication Date:
2012
detail.hit.zdb_id:
2238950-7
SSG:
22
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